丹参酚酸B对间歇性高糖乳鼠雪旺细胞损伤的改善  

Study on the protective effects of salvianolic acid B on Schwann cells injury induced by intermittent high glucose

作　　者：王蒙 王倩倩 孙连庆[1] WANG Meng;WANG Qiangqiang;SUN Lianqing(Department of Traditional Chinese Medicine,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学第一附属医院中医科,陕西西安710061

出　　处：《西部医学》2021年第10期1418-1422,共5页Medical Journal of West China

基　　金：国家自然科学基金(81673785,81874447);陕西省自然科学基础研究计划重点项目(2020JZ-34);陕西省中医药管理局项目(2019-ZZ-JC048)。

摘　　要：目的探讨丹参酚酸B(Sal B)对间歇性高糖诱导的乳鼠雪旺细胞损伤的保护作用及其机制。方法不同条件下原代培养的乳鼠雪旺细胞分为6组:正常葡萄糖组(5.6 mmol/L,Con组)、稳定性高糖组(50 mmol/L,HG组)、间歇性高糖组(正常葡萄糖与稳定性高糖交替,IHG组)及间歇性高糖加不同浓度Sal B组(25、50、100μmol/L,IHG+Sal B组)。Elisa法检测雪旺细胞培养上清丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,流式细胞仪检测细胞内活性氧(ROS)及线粒体膜电位变化,TUNNEL法检测雪旺细胞凋亡,Western blot法检测Bcl-2、Bax、AIF、活化Caspase-9及活化Caspase-3蛋白表达。结果与Con组相比,HG组与IHG组可以明显提高雪旺细胞ROS水平、MDA含量及雪旺细胞凋亡率(P<0.01),降低SOD活性及线粒体膜电位(P<0.01);上调Bax表达(P<0.01),下调Bcl-2表达(P<0.01),增加活化Caspase-9、活化Caspase-3水平及AIF的核转位(P<0.01),以上变化尤以IHG组明显(P<0.05,P<0.01)。不同浓度Sal B可以降低雪旺细胞内ROS水平及MDA含量(P<0.01),提高SOD活性及线粒体膜电位(P<0.05,P<0.01),上调Bcl-2表达(P<0.05,P<0.01),下调Bax表达(P<0.01),减少Caspase-9、Caspase-3活化及AIF的核转位(P<0.05,P<0.01),抑制雪旺细胞凋亡(P<0.05,P<0.01)。结论Sal B可有效降低间歇性高糖导致的雪旺细胞损伤,机制可能与抑制氧化应激及凋亡有关。Objective To investigate the effects of salvianolic acid B(Sal B)on the Schwann cells injury induced by intermittent high glucose(IHG)in vitro.Methods Primarily cultured SCs were randomized into normal glucose control(con),high glucose(HG),IHG,with IHG in the presence of 25μmol/L,50μmol/L,100μmol/L Sal B.After the incubation for 48 hrs,the concentration of MDA and SOD was detected by Elisa,while the ratio of apoptopic cells were detected by TUNNEL method.The levels of intracellular ROS and mitochondrial transmembrane potential were detected by flow cytometry analysis.The expression of Bcl-2,Bax,Cleaved-caspase-9,Cleaved-caspase-3 and AIF were detected by Western blot.Results The levels of intracellular ROS,MDA and the percentages of apoptotic cells were significantly increased in IHG and HG group compared with those in the control group(P<0.01).The levels of SOD and mitochondrial transmembrane potential were decreased than those in the control and HG group(P<0.01).Treatment with IHG and HG down-regulated the Bcl-2 expression(P<0.01),but up-regulated the Bax expression of protein(P<0.01).In addition,treatment with IHG and HG increased the activation of Caspase-9,Caspase-3 and the nuclear translocation of AIF in SCs(P<0.01).The injury of SCs was substantially more in cells exposed to the IHG than those exposed to HG(P<0.05,P<0.01).Moreover,the above effects of IHG were partly abolished by treatment with Sal B(P<0.05,P<0.01).Conclusion Sal B protects SCs against oxidative stress and activation of apoptopic pathway induced by IHG.

关 键 词：间歇性高糖 雪旺细胞 氧化应激 凋亡 丹参酚酸B 

分 类 号：R96[医药卫生—药理学] R285.5[医药卫生—药学]