Engagement of TLR and Dectin-1/Syk Signaling Is Required for Activation of Notch Targets in Dendritic Cells  

作　　者：Yuna Zhao Chanyang Ju Karmen Au Jimmy Zhu Baohong Zhao Yingli Shang Xiaoyu Hu 

机构地区：[1]Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,College of Veterinary Medicine,Shandong Agricultural University,Taian,Shandong,China [2]Arthritis and Tissue Degeneration Program,Hospital for Special Surgery,New York,NY,USA [3]Department of Medicine,Weill Cornell Medicine,New York,NY,USA [4]Institute for Immunology and School of Medicine,Tsinghua University,Beijing,China [5]Tsinghua-Peking Centre for Life Sciences,Beijing,China [6]Beijing Key Laboratory for Immunological Research on Chronic Diseases,Beijing,China

出　　处：《Infectious Microbes & Diseases》2021年第2期101-108,共8页感染微生物与疾病（英文）

基　　金：supported by grants from the National Natural Science Foundation of China 31725010 and 31821003(XH),grant from the Shandong Provincial Natural Science Foundation,China ZR2017MC021(YS);funds from the Tsinghua-Peking Center for Life Sciences and Institute for Immunology at Tsinghua University(XH),funds from the Peak Discipline Construction Plan of Shandong Province and Shandong Agricultural University(YS),and grants from the NIH(BZ).

摘　　要：Pattern-recognition receptors,such as toll-like receptors(TLRs),detect a wide range of microbial products and initiate innate immune responses leading to the production of inflammatory mediators.In addition,TLR signaling also activates expression of Notch target genes that play crucial roles in suppression of TLR-triggered inflammatory responses.However,whether TLR signaling pathways engaged by other classes of pattern-recognition receptors induce expression of Notch target genes remains unclear.Here we demonstrate that zymosan,a stimulus for TLR2 and dectin-1,strongly induces expression of multiple Notch target genes in both human and murine dendritic cells.Mechanistically,induction of Notch targets by zymosan is both TLR2-and Syk-dependent through activation of mitogen-activated protein kinases and the transcription factor c-Fos.Hence,our data reveals a novel mechanism that efficient induction of Notch target genes requires engagement of TLR and dectin-1/Syk signaling pathways.

关 键 词：DECTIN-1 dendritic cell Notch targets toll-like receptor zymosan Introduction 

分 类 号：R392.12[医药卫生—免疫学]