改变森林碳输入对土壤微生物磷脂脂肪酸群落组成的影响  

Effects of alterations in carbon inputs on soil microbial phospholipid fatty acid community composition in forest

作　　者：赵灿灿[1] 李印 张济麟 樊荣 马建婷 张加强 李玉洁 刘梦瑶 Zhao Cancan;Li Yin;Zhang Jilin;Fan Rong;Ma Jianting;Zhang Jiaqiang;Li Yujie;Liu Mengyao(International Joint Research Laboratory for Global Change Ecology,School of Life Sciences,Henan University,Kaifeng 475000,China)

机构地区：[1]河南大学生命科学学院,全球变化生态学国际联合实验室,河南开封475000

出　　处：《河南师范大学学报（自然科学版）》2021年第6期24-31,共8页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(U1804101);国家级大学生创新性实验训练计划项目(202010475094).

摘　　要：凋落物和植物根系是森林生态系统最重要的碳输入途径,深刻影响着土壤微生物的群落结构和功能,然而凋落物和植物根系如何影响微生物的群落结构以及其贡献仍然存在争议.以温带落叶阔叶林为研究对象,设置对照、凋落物移除、断根、断根+凋落物移除4种处理,探讨改变碳输入对土壤微生物磷脂脂肪酸群落组成的影响及内在机制.结果表明凋落物移除显著降低了微生物生物量碳和革兰氏阴性细菌比例,提高了革兰氏阳性细菌比例、革兰氏阳性细菌与阴性细菌比值、放线菌比例以及微生物生理压力,显著改变了微生物磷脂脂肪酸群落组成,而断根对微生物的群落结构没有明显影响.表明凋落物碳输入相比于植物根系对微生物磷脂脂肪酸群落组成起更重要的调控作用,凋落物主要通过改变土壤温度以及土壤碳、氮含量来调控微生物的群落组成.Litter and plant roots are the most important carbon input pathways in forest ecosystem,which profoundly affect soil microbial community composition and related functions.However,how litter and plant roots affect microbial community composition and their relative contributions are still controversial.In this study,four treatments were established including control,litter removal,root exclusion,and root exclusion plus litter removal in a temperate broadleaved deciduous forest.The objective is to investigate the effects of changing carbon inputs on soil microbial phospholipid fatty acid community composition and its internal mechanism.The results showed that litter removal significantly decreased microbial biomass carbon and percentage of Gram-negative bacteria,increased percentage of Gram-positive bacteria,the ratio of Gram-positive/Gram-negative bacteria,percentage of actinomycetes,and microbial physiological stress,which significantly changed microbial phospholipid fatty acid community composition.However,root exclusion had no obvious impact on microbial community structure.This findings suggest that litter carbon input play a more important role in regulating microbial phospholipid fatty acid community composition than plant roots.Litter regulates microbial community composition mainly by altering soil temperature and soil carbon and nitrogen content.

关 键 词：凋落物 根系 微生物群落 磷脂脂肪酸 碳输入 

分 类 号：Q938.1[生物学—微生物学]