补阳还五汤对大鼠坐骨神经横断吻合术后MAP-2,NF-M,GAP-43蛋白表达的影响  

Effect of Buyang Huanwutang on Protein Expression of MAP-2,NF-M and GAP-43 After Sciatic Nerve Transection Anastomosis in Rats

作　　者：姚思成 马贤德[1] 金连峰[2] 王春田[1] 陈崇民[3] 张锡玮[2] YAO Si-cheng;MA Xian-de;JIN Lian-feng;WANG Chun-tian;CHEN Chong-min;ZHANG Xi-wei(Liaoning University of Traditional Chinese Medicine(TCM),Shenyang 110033,China;The Affiliated Hospital of Liaoning University of TCM,Shenyang 110033,China;Shenyang Orthopaedic Hospital,Shenyang 110044,China)

机构地区：[1]辽宁中医药大学,沈阳110033 [2]辽宁中医药大学附属医院,沈阳110033 [3]沈阳市骨科医院,沈阳110044

出　　处：《中国实验方剂学杂志》2021年第20期23-30,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：沈阳市科技计划项目(18-013-0-83);辽宁省科学技术计划项目(2019JH8/10300091)。

摘　　要：目的:探讨补阳还五汤对坐骨神经横断吻合术后,大鼠坐骨神经中微管相关蛋白-2(MAP-2),神经丝蛋白(NF-M),生长相关蛋白-43(GAP-43)表达量的影响,探究补阳还五汤促进周围神经再生的机制。方法:实验选取SD大鼠作为实验对象,实验模型选取坐骨神经横断模型,随机分为模型组、假手术组、补阳还五汤组高、中、低剂量(29.6,14.8,7.4 g·kg^(-1))组、弥可保(0.156 mg·kg^(-1))组,模型组、假手术组给予等体积蒸馏水灌胃。造模成功后各组使用相应药物干预4周,4周后测试各组大鼠的坐骨神经功能指数(SFI),斜板实验度数及坐骨神经苏木素-伊红(HE)染色,并通过免疫组化和蛋白免疫印迹法(Western blot)检测各组大鼠坐骨神经吻合处的MAP-2,NF-M,GAP-43的蛋白表达。结果:与假手术组比较,模型组大鼠SFI,斜板实验度数,MAP-2,NF-M,GAP-43表达量显著增高(P<0.01);与模型组比较,补阳还五汤高、中、低剂量组SFI,斜板实验度数,MAP-2,NF-M,GAP-43表达量均明显增加(P<0.05,P<0.01)。结论:补阳还五汤对大鼠坐骨神经横断吻合术后神经再生具有十分积极的作用。Objective: To investigate the effects of Buyang Huanwutang on the expression of microtubule-associated protein-2(MAP-2),neurofilament-M(NF-M),and growth associated protein-43(GAP-43)in rat sciatic nerve after sciatic nerve transection and anastomosis. To explore the mechanism of Buyang Huanwutang promoting peripheral nerve regeneration. Method: SD rats were selected as the experimental subjects,and sciatic nerve transection model was selected as the experimental model. They were randomly divided into model group,sham operation group,Buyang Huanwutang group high,medium and low dose(29.6,14.8,7.4 g·kg^(-1))group,and mecobalamin(0.156 mg·kg^(-1))group,the model group and the sham operation group were given distilled water intragastric administration. After successful modeling,each group was treated with relevant drugs for 4 weeks. After 4 weeks,sciatic nerve function index(SFI),degree of inclined plate test and hematoxylin-eosin(HE)of sciatic nerve in each group were tested. The expression levels of MAP-2,NF-M,and GAP-43 at the sciatic nerve anastomosis site were detected by immunohistochemistry and Western blot. Result: Compared with sham operation group,the expression levels of SFI,inclined plate test,MAP-2,NF-M and GAP-43 in model group were significantly increased(P<0.01). Compared with model group,the expression levels of SFI,inclined plate test,MAP-2,NF-M and GAP-43 in Buyang Huanwutang high, medium and low-dose groups were significantly increased(P<0.05,P<0.01). Conclusion: Buyang Huanwutang has a positive effect on nerve regeneration after sciatic nerve transection and anastomosis in rats.

关 键 词：补阳还五汤 神经横断吻合术 周围神经再生 神经功能 坐骨神经 

分 类 号：R2-0[医药卫生—中医学] R33