犬Ⅰ型腺病毒Ⅸ蛋白的生物信息学分析  

Bioinformatics analysis of Canine adenovirusⅠtypeⅨprotein

作　　者：王鹏 廉士珍[1] 刘晓颖[2] 胡博 邓效禹[1] 张蕾[1] 吕爽[1] 王洪海[3] 闫喜军 朱言柱[1,2] WANG Peng;LIAN Shizhen;LIU Xiaoying;HU Bo;DENG Xiaoyu;ZHANG Lei;LYU Shuang;WANG Honghai;YAN Xijun;ZHU Yanzhu(Jilin Province Special Animal Biological Products Engineering Research Center,Institute of Special Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,China;Jilin Agricultural University,Changchun 130118,China;Heilongjiang Provice Daqing City Laboratory of Agricultural and Rural Social Service Center,Daqing 163000,China)

机构地区：[1]中国农业科学院特产研究所吉林省特种动物用生物制品工程研究中心,长春130112 [2]吉林农业大学,长春130118 [3]黑龙江省大庆市农业农村社会事业服务中心实验室,黑龙江大庆163000

出　　处：《黑龙江畜牧兽医》2021年第18期65-68,73,150,151,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：吉林省科技发展计划重点研发项目(20200402045NC)。

摘　　要：为了探究犬Ⅰ型腺病毒(CAdV-1)Ⅸ蛋白的理化性质、空间结构与抗原位点,试验以中国农业科学院特产研究所宠物生物制品研发团队实验室分离毒株的Ⅸ蛋白氨基酸序列为研究对象,采用生物信息学方法,利用ProtParam tool、NetPhos 3.1 Server、TMHMM、PSIPRED、SOPMA、Phyre 2和IEDB等在线网站对Ⅸ蛋白的理化性质、磷酸化位点、糖基化位点、跨膜区、信号肽、二级结构、三级结构、抗原决定簇和B细胞抗原决定簇进行预测。结果表明:Ⅸ蛋白含有71个氨基酸,平均吸水系数为-0.399,等电点为9.86。α-螺旋、延伸链、β-转角和无规则卷曲分别占16.90%、35.21%、9.85%和38.03%。Ⅸ蛋白不存在跨膜区和信号肽,存在2个潜在蛋白质抗原决定簇、5个蛋白质磷酸化位点,潜在B细胞抗原决定簇为18~31位。说明本试验初步预测分析得到CAdV-1Ⅸ蛋白的理化性质和抗原决定簇,可为CAdV-1Ⅸ蛋白的分离、提纯提供参考依据,同时为进一步研制基于Ⅸ蛋白的亚单位疫苗提供前期基础。In order to explore the physical and chemical properties,spatial structure and antigenic sites ofⅨprotein in Canine adenovirus typeⅠ(CAdV-1),in this study,theⅨprotein amino acid sequence of the virus that was isolated from the laboratory of the Research and Development Team of Pet Biological Products in Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,were used as the research object.Bioinformatics methods were used,and online database websites such as ProtParam tool,NetPhos 3.1 Server,TMHMM,PSIPRED,SOPMA,Phyre2 and IEDB were used to predict physicochemical property,phosphorylation site,glycosylation site,transmembrane domain,signal peptide,secondary structure,tertiary structure,antigen epitope and B cell epitope ofⅨprotein.The results showed thatⅨprotein contained 71 amino acid with an average water absorption coefficient of-0.399 and isoelectric point of 9.86.Theα-helix,extended chain,β-corner sum and random coil were 16.90%,35.21%,9.85%,and 38.03%,respectively.There was no transmembrane domain and signal peptide in the protein;there were 2 potential protein antigenic determinant,5 protein phosphorylation sites,and the potential B cell antigenic determinant was positions 18-31.The results suggested that the preliminary prediction and analysis of physicochemical properties and antigenic determinants of CAdV-1Ⅸprotein were obtained in this experiment,which could provide a reference for the isolation and purification of CAdV-1Ⅸprotein,and a preliminary basis for further development of subunit vaccine based onⅨprotein.

关 键 词：犬Ⅰ型腺病毒 Ⅸ蛋白 生物信息学 B细胞 蛋白质空间结构 抗原决定簇 

分 类 号：S852.[农业科学—基础兽医学]