射频热疗对膝骨关节炎实验兔滑膜中白细胞介素-1β及肿瘤坏死因子-α表达的影响  

The effect of radiofrequency heating on the expression of interleukin-1 beta and tumor necrosis factor alpha in osteoarthritic synovial membranes of the knee

作　　者：傅照华[1] 张飞[2] 王仙凤 孙杨梅 丁琴[1] 刘畅[1] 张冬 Fu Zhaohua;Zhang Fei;Wang Xianfeng;Sun Yangmei;Ding Qin;Liu Chang;Zhang Dong(Department of Rehabilitation Medicine,Inner Mongolia Baogang Hospital,Baotou 014010,China;Department of Orthopedics,Inner Mongolia Baogang Hospital,Baotou 014010,China)

机构地区：[1]内蒙古医科大学第三附属医院(内蒙古包钢医院)康复医学科,包头014010 [2]内蒙古医科大学第三附属医院(内蒙古包钢医院)骨科,包头014010

出　　处：《中华物理医学与康复杂志》2021年第9期776-780,共5页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：内蒙古自治区自然科学基金项目(2019MS08179)。

摘　　要：目的观察射频热疗对兔膝骨关节炎(OA)形成过程中关节软骨形态及滑膜中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)表达的影响。方法选取雄性家兔54只,采用改良Hulth造模法将其右后肢制成实验性膝OA模型。待造模成功后,将上述实验兔随机分为模型组、鹿瓜多肽组及射频热疗组。鹿瓜多肽组给予鹿瓜多肽肌肉注射,每日注射1次;射频热疗组给予射频热疗干预,选择谐振模式,温度设定为36.5~38.5℃,每日治疗1次;模型组未给予特殊处理。于治疗6 d、12 d及18 d时每组分别取6只实验兔处死,取右侧股骨内侧髁软骨,采用改良Mankins标准对其进行形态学评分,同时采用ELISA法检测各组实验兔软骨滑膜中IL-1β及TNF-α含量。结果经相同时间治疗后,发现模型组、鹿瓜多肽组及射频热疗组右侧股骨内侧髁软骨Mankins评分及滑膜中IL-1β、TNF-α含量均依次降低,组间差异均具有统计学意义(P<0.05)。射频热疗组随治疗时间延长,其右侧股骨内侧髁软骨Mankins评分及滑膜中IL-1β、TNF-α含量均逐渐降低,各观察时间点间差异均具有统计学意义(P<0.05)。结论与鹿瓜多肽肌肉注射比较,射频热疗能进一步改善膝OA实验兔股骨内侧髁软骨损伤,其治疗机制可能与调控软骨滑膜中IL-1β、TNF-α含量有关。Objective To explore the effect of radiofrequency heating on the morphology of articular cartilage in the knee and the expression of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in the synovium using a rabbit model of knee osteoarthritis(OA).Methods Fifty-four male rabbits had OA induced in their right hind limbs using the modified Hulth method.They were then randomly divided into a model group,a cervus and cucumis polypeptide(CCP)group and a radiofrequency thermotherapy(RT)group,each of 18.The CCP group was injected with deer melon peptide intramuscularly every day,while the RT group was given daily radiofrequency hyperthermia treatment at 36.5-38.5℃.The model group was not provided with any special treatment.On the 6th,12th and 18th day of the treatment,6 rabbits in each group were sacrificed to resect the right femur′s medial condyle cartilage.The morphological characteristics of the cartilage were evaluated using modified Mankins scoring,while the contents of lL-1B and TNF-a in the synovial membrane were detected using enzyme-linked immuno-sorbent assays.Results The average Mankins scores and the levels of IL-1βand TNF-αdecreased significantly at each time point,and significant differences were observed among the three groups.In the RT group the average Mankins score as well as the IL-1βand TNF-αlevels decreased significantly with time throughout the experiment.Conclusions Radiofrequency hyperthermia is superior to the injection of deer melon polypeptide in knee osteoarthritis,at least in rabbits.The therapeutic mechanism may be related to the control of IL-1βand TNF-αlevels in the synovial membrane.

关 键 词：膝骨关节炎 射频热疗 白细胞介素-1Β 肿瘤坏死因子-Α 

分 类 号：R684.3[医药卫生—骨科学] R-332[医药卫生—外科学]