广西麻鸡FOXL2基因的克隆、序列分析及在高低产蛋量卵巢中的表达  

Cloning,Sequence Analysis,and Expression Levels of FOXL2 Gene in Ovaries of High-yield and Low-yield Hens in Guangxi Indigenous Chicken

作　　者：杜文娅 夏利[1] 孙甜甜 邓继贤[2] 杨祝良[1] 唐燕飞 李淑霞 霍献强 杨秀荣[1] Du Wenya;Xia Li;Sun Tiantian;Deng Jixian;Yang Zhuliang;Tang Yanfei;Li Shuxia;Huo Xianqiang;Yang Xiurong(College of Animal Science and Technology,Guangxi University,Nanning,530004;The animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region,Nanning,530001;Guangxi Fufeng Agriculture and Husbandry Group Co.,Ltd.,Nanning,530002)

机构地区：[1]广西大学动物科学技术学院,南宁530004 [2]广西壮族自治区畜牧研究所,南宁530001 [3]广西富凤农牧集团有限公司,南宁530002

出　　处：《基因组学与应用生物学》2021年第3期980-984,共5页Genomics and Applied Biology

基　　金：广西创新驱动发展专项资金项目(桂科AA17204027)资助。

摘　　要：为了研究鸡FOXL2基因的结构和功能,本研究克隆了广西麻鸡FOXL2基因编码区序列,分析了其突变位点及与其他物种的同源性和进化距离,以及在高产组和低产组母鸡卵巢组织中的表达水平。结果表明:广西麻鸡FOXL2基因的编码区长度为918 bp,共编码305个氨基酸,存在一处错义突变和两处同义突变。通过物种间的同源性分析显示,广西麻鸡FOXL2基因与原鸡(Gallus gallus)、雉鸡(Phasianus colchicus)、绿头鸭(Anas platyrhynchos)、野鸽(Columba livia)、人(Homo sapiens)、小鼠(Mus musculus)的同源性分别为99.7%、98.8%、95.1%、92.0%、75.7%、75.5%。通过种间进化树分析表明,广西麻鸡与原鸡(Gallus gallus)的亲缘关系最近,与小鼠(Mus musculus)的亲缘关系最远。FOXL2在高低产蛋量鸡卵巢中的表达水平结果显示:FOXL2基因在高产蛋组中的表达量显著高于低产蛋组中的表达量(P<0.05)。该研究结果提示鸡FOXL2的序列相对较保守,对卵巢功能的维持和提高产蛋量具有功能性作用。To study the structure and function of the chicken FOXL2 gene,in this study,the FOXL2 gene coding region sequence of Guangxi indigenous chicken was cloned.The mutation site,homology,and evolutionary distance with other species,aswell as the expression level of FOXL2 gene in ovarian tissues of hens in high-yield and low-yield groups were analyzed.The results showed that there was a FOXL2 gene coding region sequence of 918 bp in Guangxi indigenous chicken,encoding 305 amino acids,with one missense mutation and two synonymous mutations.The homology analysis showed that the homology of FOXL2 gene of Guangxi indigenous chicken with FOXL2 gene of Gallus gallus,Phasianus colchicus,Anas platyrhynchos,Columba livia,Homo sapiens,and Mus musculus were 99.7%,98.8%,95.1%,92.0%,75.7%,and 75.5%.Phylogenetic tree analysis showed that FOXL2 gene in Guangxi indigenous chicken had the closest relationship with FOXL2 gene in Gallus gallus and the farthest relationship with FOXL2 gene in Mus musculus.The expression level of FOXL2 gene in ovaries tissues of high-yield and low-yield hens showed that the expression level of FOXL2 gene in the high-yield group was significantly higher than that in the low-yield group(P<0.05).The results of this study suggested that the sequence of FOXL2 gene in chickenswas relatively conservative and of functions for maintaining ovarian function and improving egg production.

关 键 词：鸡 产蛋性能 FOXL2 基因克隆 组织表达 

分 类 号：S831[农业科学—畜牧学]