山羊FGF5基因组织表达及其变异与羊绒性状的关联性分析  

Analysis of Tissue Expression of Goat FGF5 Gene and Association Between the Gene Variation and Cashmere Traits

作　　者：张瑞国 何兆华 王继卿 刘秀 罗玉柱 李少斌 Zhang Ruiguo;He Zhaohua;Wang Jiqing;Liu Xiu;Luo Yuzhu;Li Shaobin(Gansu Key Laboratory of Herbivorous Animal Biotechnology,College of Animal Science and Technology,Gansu Agricultural University,Lanzhou,730070)

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃省草食动物生物技术重点实验室,兰州730070

出　　处：《基因组学与应用生物学》2021年第3期1007-1014,共8页Genomics and Applied Biology

基　　金：甘肃省创新群体计划项目(18JR3RA190);甘肃省草食动物生物技术重点实验室开放课题(GKLAB-201702);甘肃农业大学创新基金项目(GSAU-ZL-2015-033);甘肃省高等学校创新能力提升项目(2019A-050);甘肃农业大学伏羲人才计划(Gaufx-03Y04)共同资助。

摘　　要：为了研究山羊FGF5基因的遗传变异和组织表达等分子特征及其变异对山羊羊绒性状的影响,本试验应用PCR-SSCP与测序相结合的方法检测FGF5基因第1外显子在陇东绒山羊、柴达木绒山羊和中卫山羊中的多态性,并分析该基因变异对羊绒性状的影响;采用RT-qPCR技术研究FGF5基因在陇东绒山羊和辽宁绒山羊组织中的表达。结果表明,在山羊FGF5基因的第1外显子区检测到一个同义突变位点c.272C>T,表现为MM、MN和NN 3种基因型,其中NN型仅在陇东绒山羊中有极少量分布。3个山羊品种的优势基因型均为MM,优势等位基因均为M。c.272C>T位点在3种山羊品种中均处于Hardy-Weinberg平衡状态,且均为低度多态。c.272C>T位点与陇东绒山羊的羊绒性状关联性分析表明,该位点对羊绒纤维直径的影响显著。携带等位基因N的个体的羊绒纤维直径显著低于不含等位基因N的个体的(P<0.05);基因型MN个体的羊绒纤维直径显著低于基因型MM个体的(P<0.05)。FGF5基因在陇东绒山羊和辽宁绒山羊的心、肝、脾、肺、肾、骨骼肌和皮肤中均有表达;在陇东绒山羊的心、脾、肺、肾和皮肤中的表达量显著或极显著高于辽宁绒山羊的。本研究结果提示,若以改善羊绒细度为选育目标,山羊FGF5基因可作为羊绒性状改良的候选基因。To investigate the molecular characteristics including gene variation and tissue expression of goat FGF5 gene and effect of its variation on cashmere traits in goat,PCR-SSCP combined with sequencing were used to detect the polymorphism of exon 1 of FGF5 gene in Longdong Cashmere goats,Chaidamu Cashmere goats and Zhongwei goats,and analyze the effect of FGF5 gene variation on cashmere traits were analyzed.RT-qPCR technique was used to study the expression of FGF5 gene in tissues of Liaoning Cashmere goats and Longdong Cashmere goats.The result showed that there was a synonymous SNP site c.272C>T in the exon 1 of goat FGF5 gene,and there were three genotypes,named MM,MN,and NN,respectively.NN was rare and only detected in Longdong Cashmere goats.All the dominant genotypes and alleles of the three goat breeds were MM and M.c.272C>T was in Hardy-Weinberg equilibrium among the three goat breeds,and all of them showed low polymorphism.The association analysis between c.272C>T and cashmere traits of Longdong Cashmere goats showed that this SNP had a significant effect on cashmere fiber diameters.The cashmere fiber diameter of individuals with N was significantly lower than that of individuals without N(P<0.05);The cashmere fiber diameter of individuals with MN genotype was significantly lower than that of individuals with MM genotype(P<0.05).The FGF5 gene was expressed in the heart,liver,spleen,lung,kidney,skeletal muscle and skin of Liaoning Cashmere goats and Longdong Cashmere goats;the expression in the heart,spleen,lung and kidney and skin of Longdong Cashmere goats was significantly higher than that of Liaoning Cashmere goats.These results suggested that goat FGF5 gene could be used as a candidate gene for breeding programs based on improving fibre diameter.

关 键 词：FGF5 陇东绒山羊 羊绒性状 纤维直径 

分 类 号：S827[农业科学—畜牧学]