栽培绿狐尾藻的鱼塘底泥菌群结构与优势属生态位特征分析  

Analysis of Bacterial Community Structure and Niche Characteristics of the Fishpond Sediment Under the Cultivation of Myriophyllum elatinoides

作　　者：徐紫娟 李文红 武利军 陈祯东 张宇航 杨卓 徐鹏 温晴宇 Xu Zijuan;Li Wenhong;Wu Lijun;Chen Zhendong;Zhang Yuhang;Yang Zhuo;Xu Peng;Wen Qingyu(Key Laboratory of Guangxi Colleges and Universities for Aquatic Healthy Breeding and Nutrition Regulation,College of Animal Science and Technology,Guangxi University,Nanning,530004)

机构地区：[1]广西大学动物科学技术学院,广西高校水生生物健康养殖与营养调控重点实验室,南宁530004

出　　处：《基因组学与应用生物学》2021年第3期1196-1204,共9页Genomics and Applied Biology

基　　金：广西科技创新驱动专项项目(桂科AA17204095-2);国家现代农业产业技术体系广西罗非鱼创新团队建设项目(nycytxgxcxtd-08-03)共同资助。

摘　　要：为探究栽培绿狐尾藻的鱼塘底泥细菌组成以及优势菌属的生态位特征,本研究采用高通量测序技术检测底泥细菌在门水平和属水平的组成,计算优势菌属的生态位宽度、生态位重叠指数,并分析了优势属和环境理化因子之间的关系。结果表明,栽培绿狐尾藻的鱼塘底泥细菌归属于63个门类、153个纲、190个目、168个科、143个属;6门15优势属以变形菌门为第一大门类,其次为绿弯菌门、拟杆菌门、酸杆菌门、硝化螺旋菌门、OP8门等。15个优势属的生态位宽度介于0.584~0.957,80%的优势属生态位宽度大于0.8;优势属生态位重叠值的变化范围为0.505~0.983,生态位重叠值大于0.6的属占优势属总对数的98.0%;表明各优势属的资源利用和生存能力较强,存在激烈资源竞争,空间异质性较小。冗余分析发现上覆水总磷、氨氮浓度和底泥有机质是影响优势菌属丰度的主要环境因子。本研究结果为揭示绿狐尾藻对淡水养殖池塘环境微生物的影响提供科学依据。To furth er u nderstand the microbial structure and niche characteristics of sediment in the ponds planted with Myriophyllum elatinoides,high-throughput sequencing technology was used in this study to analyze the structure of bacteria and niche characteristics of dominant bacteria from the composition of bacteria at the level of phylum and genus.Niche width of dominant genera and niche overlap index were calculated,and the relationship between dominant genera and environmental physicochemical factors was analyzed in this paper.The results showed that the bacteria in the sediment of fishpond planted with Myriophyllum elatinoides belong to 63 phyla,153 classes,190 orders,168 families and 143 genera,and 15 dominant generas of bacteria of 6 phyla mainly concentrated in Proteobacteria,followed by Chloroflexi,Bacteroidetes,Acidobacteria,Nitrospira and OP8 etc.The niche width of 15 dominant genera ranged from 0.584 to 0.957,80%of which was larger than 0.8.The range of niche overlap value of dominant genera was 0.505~0.983,and those with niche overlap value greater than 0.6 accounted for 98.0%of the total logarithm of dominant genera.Niche breadth and overlap value showed that the resource utilization and survival ability of the dominant genera were strong.There was fierce resource competition,and the spatial heterogeneity was small.The redundancy analysis result showed that the concentrations of TP,Ammonia and OM were the main environmental factors,which affected the abundance of dominant bacteria.The results of this study provided a scientific basis for revealing the effects of Myriophyllum elatinoides on the environmental microorganisms in freshwater aquaculture ponds.

关 键 词：鱼塘底泥 菌群结构 优势属 生态位 绿狐尾藻 

分 类 号：Q948.1[生物学—植物学]