茅苍术GPS基因(AlGPS)的克隆与序列分析  

Cloning and Sequence Analysis of GPS Gene(AlGPS)from Atractylodes lancea

作　　者：毛逸桂 陈丽娜 张爱松 王可 陆勇 龚玲[1] 余坤[1] Mao Yigui;Chen Lina;Zhang Aisong;Wang Ke;Lu Yong;Gong Ling;Yu Kun(College of Pharmacy,Hubei University of Chinese Medicine,Wuhan,430065)

机构地区：[1]湖北中医药大学药学学院,武汉430065

出　　处：《基因组学与应用生物学》2021年第3期1300-1306,共7页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31670341,31300277);湖北省技术创新专项(2018ACA124)共同资助。

摘　　要：为获得茅苍术(Atractylodes lancea)GPS基因全长及序列特征,本研究根据转录组测序结果,用特异性引物进行PCR扩增,得到茅苍术GPS(AlGPS)开放阅读框全长共1266 bp(GenBank登录号:MH522714.1),编码421个氨基酸。生物信息学分析结果表明,AlGPS氨基酸序列与青蒿的亲缘关系最近,属异戊二烯超家族成员和牻牛儿基焦磷酸合酶超家族成员;AlGPS蛋白为非分泌蛋白,跨膜结构预测为膜内在蛋白;亚细胞定位显示该蛋白很可能存在于叶绿体。本研究获得的AlGPS基因序列及序列特征分析可为茅苍术萜类化合物生物合成及调控奠定基础,并为进一步阐明AlGPS的生物学功能和分子育种提供科学依据。This study aimed to obtain the full length of geranyl diphosphate synthase(GPS)gene(AlGPS)from Atractylodes lancea,and clarify its structural characteristics.The specific primers were generated based on the previous trancriptome sequences,and used for PCR amplification to obtain the full length cDNA sequence of AlGPS.The open reading frame of AlGPS was 1266 bp encoding 421 amino acids(GenBank accession number:MH5227-14.1).The results of bioinformatics analysis showed that the AlGPS had the highest similarity to the GPS from Artemisia annua,both of which contained the domain of isoprenoid synthase superfamily and geranyl diphosphate synthase superfamily.AlGPS protein was non-secreted protein,and its transmembrane structure was predicted to be intramembrane protein.Subcellular localization indicated that the protein was probably present in the chloroplast.The AlGPS gene sequences and biological characteristics obtained in this study provide references for terpene biosynthesis and regulation in Atractylodes lancea,and will further facilitate the study on the biological function of AlGPS and molecular breeding in A.lancea.

关 键 词：GPS基因 茅苍术 基因克隆 生物信息学分析 

分 类 号：S567.239[农业科学—中草药栽培]