碳氮源优化提高兼养三角褐指藻生物量和岩藻黄素产量  

Improving Production of Biomass and Fucoxanthin in Mixotrophic Phaeodactylum tricornutum by Optimization of Carbon and Nitrogen Sources

作　　者：王珊 杨润青 宋培钦 魏东[1] WANG Shan;YANG Runqing;SONG Peiqin;WEI Dong(School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640

出　　处：《食品与生物技术学报》2021年第10期82-90,共9页Journal of Food Science and Biotechnology

基　　金：广东省公益研究与能力建设项目(2015A020216003)。

摘　　要：岩藻黄素(Fucoxanthin)是一种参与海洋藻类光合作用的类胡萝卜素,具有抗氧化、抗癌、抗肥胖和抗糖尿病等多种功效,作为生物医药和功能性食品具有重要应用价值。海洋硅藻三角褐指藻含有高含量岩藻黄素,被认为是褐藻之外的藻基岩藻黄素的新来源。为提高岩藻黄素产量,作者以三角褐指藻(Phaeodactylum tricornutum CCMP2561)为研究对象,系统评价了兼养(光发酵)条件下有机碳源、氮源种类和浓度对其生物量和岩藻黄素产量的影响,筛选出最优碳氮源组合为0.10 mol/L甘油和含氮量为0.02 mol/L的胰蛋白胨与尿素(浓度比1∶1)混合氮源。采用此优化组合,在光照强度2000 lx、培养温度20℃、摇床转速150 r/min条件下,可获得三角褐指藻生物量、岩藻黄素质量分数和产量最大值,分别为3.94 g/L、20.83 mg/g和81.97 mg/L,是优化前的3.15倍、11.64倍和36.59倍。本研究开发的优化兼养培养基和培养策略,为显著提高三角褐指藻岩藻黄素产量提供了实用新技术。Fucoxanthin is a kind of carotenoids that participates in the photosynthesis of marine algae and has multiple functions such as anti-oxidation,anti-cancer,anti-obesity and anti-diabetes,showing important applications value as the biological medicine and functional food.Marine diatom Phaeodactylum tricornutum has high content of fucoxanthin,which is considered as a new source of algae-based fucoxanthin besides brown seaweed.Aiming to improve the fucoxanthin production,P.tricornutum CCMP2561 was applied in this study to systematically evaluate the effects of organic carbon,nitrogen sources and their concentrations on the cell growth and fucoxanthin production under mixotrophic(photo fermentation)conditions.The optimal combination of carbon and nitrogen source was 0.10 mol/L of glycerol,0.02 mol/L of total nitrogen mixed by tryptone and urea(1∶1).Under this optimal combination,the maximum biomass concentration,fucoxanthin content and yield were achieved at 3.94 g/L,20.83 mg/g and 81.97 mg/L in P.tricornutum respectively,which were3.15,11.64,and 36.59 times to those before optimization under 2000 lx of light intensity,20℃of culture temperature and 150 r/min of shaking speed.The optimal medium and approach for mixotrophic cultivation developed in this study could provide a newly practical technology for significant enhancement of fucoxanthin production in P.tricornutum.

关 键 词：三角褐指藻 兼养 甘油 氮源 岩藻黄素 

分 类 号：Q920.1[生物学]