Fe_(3)O_(4)纳米粒子的仿生修饰及叶酸靶向功能化研究  

Study on the Surface Modification of Dopamine and Folic Acid Molecule on Superparamagnetic Fe_(3)O_(4) Nanoparticles

作　　者：刘晓迪 张寒思 宋新峰 LIU Xiao-di;ZHANG Han-si;SONG Xin-feng(College of Medicine and Nursing,Dezhou University,Dezhou 253023,China;Graduate School,East China Normal University,Shanghai 200062,China)

机构地区：[1]德州学院医药与护理学院,山东德州253023 [2]华东师范大学研究生院,上海200062

出　　处：《广州化学》2021年第5期36-42,共7页Guangzhou Chemistry

基　　金：国家大学生创新创业训练计划项目(201510448055);山东省大学生创新创业训练计划项目(S201910448029)。

摘　　要：采用共沉淀法制备超顺磁性Fe_(3)O_(4)纳米粒子(SPIONs),对SPIONs进行多巴胺仿生修饰制备SPIONs@PDA;在此基础上键合靶向分子叶酸制备SPIONs@PDA-FA。用扫描电镜、马尔文粒度仪、振动样品磁强计、差示—热重分析仪以及傅里叶红外光谱等对其形貌、粒径大小及分布、磁学性能、化学组成等进行了表征。结果表明,扫描电镜显示SPIONs@PDA-FA为近似球形,分散性好,具有较好的超顺磁性;键合叶酸的SPIONs能明显增强巨噬细胞的内吞效应。Dopamine-coated superparamagnetic Fe_(3)O_(4) nanoparticles(abbreviated as SPIONs@PDA)were prepared by a facile and simple approach.Then the folic acid(FA)molecule was conjugated to SPIONs@PDA with the carboxyl group to preferentially target cancer cells with folate receptors expressed on their membranes and facilitate the transit of the nanoparticles across the cell membrane.The morphology,particle size,distributed magnetic properties and chemical composition of the samples were characterized separately by scanning electron microscopy,Malvin particle size analyzer,vibration sample magnetometer,differential thermogravimetric analyzer and Fourier transform infrared spectroscopy.As a result,SPIONs@PDA-FA exhibited relatively high saturation magnetization and good dispersibility.The results in vitro show that SPIONs@PDA-FA appear to be the enrichment in RAW264.7 cells.

关 键 词：磁性纳米粒子 仿生修饰 多巴胺 叶酸 磁共振成像造影剂 

分 类 号：O69[理学—化学]