利用近红外-吲哚菁绿成像系统判断急性肠缺血模型缺血肠段的实验研究  

Application of near infrared-indocyanine green imaging system for judging ischemic intestinal segment in a canine model of acute intestinal ischemia

作　　者：潘力迦 吴晔明[1] 王奕[1] 侯光辉 Pan Lijia;Wu Yeming;Wang Yi;Hou Guanghui(Department of Pediatric Surgery,Affiliated Xinhua Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai,200092,China;Guangdong OptoMedic Technologies Inc,Guangzhou 528200,China)

机构地区：[1]上海交通大学医学院附属新华医院儿外科,上海市200092 [2]广东欧谱曼迪科技有限公司,广东省广州市528200

出　　处：《临床小儿外科杂志》2021年第10期925-929,共5页Journal of Clinical Pediatric Surgery

基　　金：国家自然科学基金(编号:81874234);苏州市临床医学专家团队引进项目(编号:SZYJTD201706);上海市市级医院新兴前沿技术联合攻关项目(编号:SHDC12018123);上海市生物医药领域科技支撑项目(编号:18401931800);上海市转化医学协同创新中心项目(编号:TM201809)。

摘　　要：目的近红外-吲哚菁绿(near infrared-indocyanine green,NIR-ICG)成像系统可通过吲哚菁绿(indocyanine green,ICG)示踪血液流动情况,有望应用于肠管血运状况的评估。本研究利用犬动物模型模拟急性肠管血液循环障碍,以此评估NIR-ICG成像系统在手术中检测缺血肠段的价值。方法无损夹闭实验犬的一段空肠或回肠系膜,并阻断其间分布的所有空肠动静脉与回肠动静脉分支,建立急性肠缺血模型。静脉注射ICG,追踪实验犬不同时间点肠段显影强度变化,利用系统自带软件进行实时半定量分析。结果建立急性肠缺血模型后注射ICG(1 mg/kg)并等待1 min后,缺血肠段没有显影,但周围正常肠段已经清晰成像,定义正常肠段荧光强度为100%,缺血肠段荧光强度为0%;3 min后缺血肠段荧光强度为0%。显影后建立再灌注损伤模型;定义0 min时荧光强度为100%,120 min后再灌注损伤肠段荧光强度为20.3%,而周围正常肠段荧光强度下降为0%。结论利用NIR-ICG成像系统可快速分辨出缺血肠段,为术中判断肠段缺血提供了一种客观、准确的新方法。Objective To apply near infrared-indocyanine green(NIR-ICG)imaging system can assess intestinal blood supply through tracing blood flow with indocyanine green(ICG)in a canine model of acute intestinal blood supply disorder.Methods An acute ischemic intestinal model was established by non-destructively clamping a section of canine jejunal/ileal mesentery and blocking all relevant branches of jejunal and ileal blood vessels.After an intravenous injection of ICG,the changes of intestinal fluorescent intensity were tracked at different timepoints and the system's software was utilized for real-time semi-quantitative analysis.Results ICG 1 mg/kg was injected after modeling.After 1 min,ischemic intestine remained invisible while adjoining normal intestine was distinctly visualized.Fluorescent intensity of normal intestine was defined as 100%and ischemic intestine as 0%.After 3 min,fluorescent intensity of ischemic intestine was still 0%.Then reperfusion injury model was established with an injection of ICG fluorescent intensity was defined as 100%at 0 min.After 120 min,fluorescent intensity of injured intestinal segment declined to 20.3%while adjoining normal intestinal segment dropped to 0%.Conclusion Capable of distinguishing ischemic intestinal segments quickly,NIR-ICG imaging system provides an objective and accurate new tool for judging intestinal ischemia during surgery.

关 键 词：荧光染料 吲哚菁绿 诊断显像 急性肠缺血 再灌注损伤 动物模型 

分 类 号：R917.76[医药卫生—药物分析学] R574[医药卫生—药学]