休哈塔假丝酵母(Candida shehatae)HDYXHT-01利用玉米芯半纤维素水解液发酵产乙醇的研究  

Study on ethanol production from Candida shehatae HDYXHT-01 using corncob hemicellulose hydrolysate

作　　者：李娜 任焱鑫 田甜 杨晓峰[1] 葛菁萍[1,2] LI Na;REN Yanxin;TIAN Tian;YANG Xiaofeng;GE Jingping(Key Laboratory of Microbiology,College of Life Sciences,Heilongjiang University,Harbin 150080,China;Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500,China)

机构地区：[1]黑龙江大学生命科学学院微生物省高校重点实验室,哈尔滨150080 [2]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500

出　　处：《黑龙江大学自然科学学报》2021年第4期468-474,共7页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(32071519);黑龙江省自然科学基金重点资助项目(ZD2020C008)。

摘　　要：通过对休哈塔假丝酵母(Candida shehatae)HDYXHT-01的耐水解液毒性驯化,筛选出一株具有较高乙醇生产强度以及较高水解液毒性物质耐受能力的驯化菌株C.shehatae YHX-9。乙醇生产强度为0.495 g·L^(-1)·h^(-1),是出发菌株(0.426 g·L^(-1)·h^(-1))的1.16倍,乙酸耐受极限为4 g·L^(-1),糠醛耐受极限为5 g·L^(-1),分别较驯化前提高了1.33倍和1.25倍。为了进一步提高该菌株发酵玉米芯半纤维素水解液产乙醇的能力,对水解液发酵条件进行优化。优化后的条件为:水解液浓缩倍数为3倍(含葡萄糖5.12 g·L^(-1),木糖68.7 g·L^(-1),阿拉伯糖10.19 g·L^(-1)),装液量为60 mL/250 mL,发酵时间为64 h,pH为5.4,接种量为8%。经过优化,乙醇平均产量达到了27.01 g·L^(-1),是未经工艺优化前最高产量23.90 g·L^(-1)的1.13倍,为进一步利用C.shehatae进行乙醇生产奠定了坚实的基础。The toxicity of hydrolysis resistance agent of Candida shehatae HDYXHT-01 was acclimatized to screen an acclimated bacterium C.shehatae YHX-9 which had high production intensity of ethyl alcohol and high tolerance to hydrolysate toxicity substances.The production intensity of ethyl alcohol was 0.495 g·L^(-1)·h^(-1),which was 1.16 times of that of original strain(0.426 g·L^(-1)·h^(-1)).The tolerance limit of acetic acid and furfural was 4 g·L^(-1) and 5 g·L^(-1),respectively,which was 1.33 times and 1.25 times of that before acclimatization,respectively.In order to further improve the ethyl alcohol production capacity of strain fermented corncob hemicellulose hydrolysate,the fermentation conditions of hydrolysate were optimized.The conditions after optimization were as below,concentration multiple of hydrolysate was 3(containing 5.12 g·L^(-1) glucose,68.7 g·L^(-1) xylose and 10.19 g·L^(-1) arabinose),the loaded liquid,fermentation time,pH value and inoculum size were 60 mL/250 mL,64 h,5.4 and 8%,respectively.After optimization,the average yield of ethyl alcohol reached 27.01 g·L^(-1),which was 1.13 times of maximum yield(23.90 g·L^(-1))before process optimization,laying a solid foundation to further utilize C.Shehatae for ethyl alcohol production.

关 键 词：乙醇 休哈塔假丝酵母 玉米芯半纤维素 驯化 正交试验设计 

分 类 号：Q939.97[生物学—微生物学]