头孢菌素C产生菌的诱变育种及发酵应用  

Mutation Breeding and Fermentation Application of Cephalosporin C-Producing Strain Cephalosporium acremonium

作　　者：牛李杰 张婷 党建宁 张宝新 NIU Lijie;ZHANG Ting;DANG Jianning;ZHANG Baoxin(Yili Chuanning Biotechnology Co.,Ltd.,Yining 835000,China)

机构地区：[1]伊犁川宁生物技术股份有限公司,新疆伊宁835000

出　　处：《化学与生物工程》2021年第10期57-61,共5页Chemistry & Bioengineering

摘　　要：以头孢菌素C产生菌顶头孢霉(Cephalosporium acremonium)B-1822-S-04为出发菌株,经过紫外线和丙二酸复合诱变,筛选得到了4株头孢菌素C高产菌株D-G-017、D-G-083、D-G-096和D-G^(-1)33,其中菌株D-G-096的效价高达6816 mg·L^(-1),较出发菌株提高了50.80%,且高产特性稳定;50 L发酵罐小试结果显示,菌株D-G-096的效价达到了29270 mg·L^(-1),较出发菌株提高了111.06%,可作为优良高产菌株保存使用。该实验筛选出的高产菌株及其在小试中的应用,可为后期工业化生产提供优质菌种资源,为发酵工艺提供指导。Using a Cephalosporin C-producing strain Cephalosporium acremonium B-1822-S-04 as the starting strain,we screened four Cephalosporin C high-yield strains D-G-017,D-G-083,D-G-096,and D-G^(-1)33,with compound mutagenesis by UV and malonic acid.The titer of strain D-G-096 is as high as 6816 mg·L^(-1),which is 50.80%higher than that of the starting strain,and the high-yield characteristics is stable.The small-scale experiments in 50 L fermentor show that the titer of the strain D-G-096 reaches 29270 mg·L^(-1),which is 111.06%higher than that of the starting strain.It can be used as an excellent high-yield strain.The high-yield strains screened in this experiment and their application in small-scale experiment can provide high-quality strain resources and fermentation process guidance for later industrial production.

关 键 词：紫外线 丙二酸 复合诱变 头孢菌素C 顶头孢霉 

分 类 号：TQ465[化学工程—制药化工]