Daxx对γ射线诱导HeLa细胞凋亡的影响  

Effects of Daxx onγ-ray induced apoptosis of HeLa cells

作　　者：唐双阳[1] 丁霜 申海艳[1] 伍虹蓉 胡煜 廖雅琪 熊雨棱 李乐[2] TANG Shuangyang;DING Shuang;SHEN Haiyan;WU Hongrong;HU Yu;LIAO Yaqi;XIONG Yuling;LI Le(Pathogenic Biology Institute,Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control,University of South China,Hengyang 421001,China;School of Public Health,University of South China,Hengyang 421001,China;Hengyang Medicine School,University of South China,Hengyang 421001,China)

机构地区：[1]南华大学病原生物学研究所特殊病原体防控湖南省重点实验室,衡阳421001 [2]南华大学公共卫生学院,衡阳421001 [3]南华大学衡阳医学院,衡阳421001

出　　处：《辐射研究与辐射工艺学报》2021年第5期55-60,共6页Journal of Radiation Research and Radiation Processing

基　　金：湖南省教育厅青年项目(18B269);湖南省卫生健康委科研重点项目(202105012137);湖南省大学生创新创业训练计划项目(湘教通[2020]191号No.2683);衡阳市科技计划项目(202002042122);国家自然科学基金项目(81402169);湖南省自然科学基金项目(2018JJ3452、202150014);特殊病原体防控湖南省重点实验室资助项目(湘科计字[2014]5号)资助。

摘　　要：研究过表达死亡结构域相关蛋白(Daxx)对经γ射线照射过的细胞凋亡的影响,探讨Daxx与辐射诱导细胞凋亡之间的关系及其机制。通过转染将宫颈癌HeLa细胞分为正常对照组、空质粒转染阴性组与Daxx转染组,利用生物细胞辐照仪经γ射线照射至不同剂量;Western blot检测细胞中Daxx的表达;MTT检测细胞的增殖情况;ELISA检测细胞中Caspase-8的相对活性;流式细胞术检测细胞凋亡情况。结果发现:随着照射剂量增加,细胞数量逐渐减少,且其中变形细胞越来越多;Daxx表达水平随着照射剂量增加而升高;Daxx转染的辐照细胞组的细胞增殖抑制率、A 405值以及细胞凋亡率均明显高于未辐照细胞组,差异均有统计学意义(p<0.01,p<0.05,p<0.05)。过表达Daxx能通过上调经γ射线照射细胞的Caspase-8活性,促进辐射诱导的细胞凋亡。We studied the effects of overexpression of deathdomainassociated protein(Daxx)on cell apoptosis underγ-ray radiation.Further,we explored the relationship between Daxx and radiation-induced apoptosis and investigated the mechanism behind such apoptosis.Through transfection,cervicalcancer HeLa cells were divided into a control group,an empty plasmid-transfected negative group,and a Daxx-transfected group;the HeLa cells were irradiated with different doses ofγ-rays using a cell irradiator.The expression of Daxx protein in cells was detected by Western blot.Cell proliferation was detected by MTT.The relative activity of caspase-8 in cells was detected by ELISA whereas the cell apoptosis was detected by flow cytometry.The results showed that the total number of cells decreased with the increase in absorbed dose,whereas the number of deformed cells increased.Furthermore,the degree of Daxx expression increased with the increase in absorbed dose.Comparing the cells of the Daxx-transfected group with those of the unirradiated control group,we observed statistically significant increments(p<0.01,p<0.05,p<0.05)in the rate of inhibition against cell proliferation,the A 405 value,and rate of apoptosis.Hence,overexpression of Daxx promotes radiation-induced apoptosis by upregulating caspase-8 activity in theγ-ray irradiated cells.

关 键 词：死亡结构域相关蛋白(Daxx) 凋亡 电离辐射 

分 类 号：R811[医药卫生—放射医学]