静脉注射旱莲苷A的药动学研究  

Pharmacokinetics of ecliptasaponin A after intravenous administration

作　　者：石碧茹 高建[1] 赵晶[1] 刘二伟[1] 高秀梅[1] 韩立峰[1] SHI Bi-ru;GAO Jian;ZHAO Jing

机构地区：[1]天津中医药大学组分中药国家重点实验室,天津市中药化学与分析重点实验室,天津301617

出　　处：《中南药学》2021年第10期2059-2062,共4页Central South Pharmacy

基　　金：天津市科技计划资助项目(编号:20ZYJDJC00120)。

摘　　要：目的研究旱莲苷A经尾静脉注射后的药动学特征。方法基于高效液相色谱-串联三重四极杆质谱平台(HPLC-QQQ-MS)对供试品进行分析,采用Agilent Eclipse Plus C_(18)(2.1 mm×150 mm,5 μm)色谱柱分离;流动相为乙腈(A)-0.05%乙酸水(B),梯度洗脱;流速0.4 mL·min^(-1) ,柱温25℃;进样量5 μL。多反应离子监测模式检测目标化合物。通过方法学考察建立大鼠静脉给药旱莲苷A后的药动学分析方法,DAS软件处理药动学参数。结果大鼠经尾静脉注射旱莲苷A后,药动学参数符合二室开放药动学模型,平均达峰时间t_(max)为0.0833 h、达峰浓度C_(max)为131.33 ng·mL^(-1) 、药时曲线下面积AUC_(0~∞)为142.72 h·ng·mL^(-1) 、半衰期t_(1/2)α是0.1106 h、半衰期t_(1/2)β是3.0635 h、清除速率CL为0.0117 L·h^(-1) 。结论 该方法,灵敏度高、准确性好,能够用于旱莲苷A药动学研究。Objective To determine the pharmacokinetic parameters of ecliptasaponin A after intravenous administration.Methods The bio-samples were analyzed by HPLC-QQQ-MS.The samples were separated on an Agilent Eclipse Plus C18(2.1 mm×150 mm,5μm)column and were eluted by acetonitrile-0.05%acetic acid gradiently.The flow rate was 0.4 mL•min^(-1),and the column temperature was 25℃.MRM mode was used to detect the target compounds.Pharmacokinetics analytical of ecliptasaponin A after intravenous administration was established.DAS software was used to process the pharmacokinetic parameters.Results The pharmacokinetic parameters of ecliptasaponin A were in accordance with the two compartment open model.The mean tmax was 0.0833 h,and the Cmax was 131.33 ng·mL^(-1).The AUC0~∞was 142.72 h·ng·mL^(-1),and the t1/2αand t1/2βwas 0.1106 h and 3.0635 h,respectively.The clearance rate was 0.0117 L·h^(-1).Conclusion This method is sensitive and accurate,and is suitable for the pharmacokinetic study of ecliptasaponin A.

关 键 词：墨旱莲 旱莲苷A 高效液相色谱-串联三重四极杆质谱平台 药动学 

分 类 号：R965[医药卫生—药理学]