高效液相色谱-蒸发光散射检测器法同时测定舞阳贝母中3种生物碱类成分的含量  

Determination of 3 alkaloids in Fritillaria Wuyangensis by HPLC-ELSD

作　　者：李珊 王晓燕[1] 张红伟 李向阳[1] 刘菊 黄霞 LI Shan;WANG Xiao-yan;ZHANG Hong-wei;LI Xiang-yang;LIU Ju;HUANG Xia(Henan Institute for Food and Drug Control,NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine(Chinese Materia Medica and Prepared Slices),Zhengzhou 450018;Zhengzhou Hospital of Traditional Chinese Medicine,Zhengzhou 450007)

机构地区：[1]河南省食品药品检验所国家药品监督管理局中药材及饮片质量控制重点实验室,郑州450018 [2]郑州市中医院,郑州450007

出　　处：《中南药学》2021年第10期2158-2161,共4页Central South Pharmacy

基　　金：河南省科技攻关项目(No.202102310189);河南省市场监督管理局科技计划项目(No.2020sj26)。

摘　　要：目的建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法同时测定舞阳贝母中3种生物碱类成分(贝母辛、贝母素甲、贝母素乙)的含量。方法采用YMC-Triart C_(18)色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈和0.1%三氟乙酸,梯度洗脱,流速1.0 mL·min^(-1) ,柱温30℃,检测器ALLTECH2000 ES ELSD,漂移管温度105℃,载气流速2.5 L·min^(-1) 。结果贝母辛、贝母素甲、贝母素乙的线性范围分别为0.0409~0.4092、0.0499~0.4986、0.0421~0.4208 mg·mL^(-1) ,r均≥0.9991;平均加样回收率分别为97.6%、96.3%、98.8%,RSD均≤1.9%。结论本法操作简便,重复性好,准确可靠,可用于舞阳贝母中3种生物碱类成分的含量测定。Objective To determine 3 alkaloids(peimisine,verticine and verticinone from Fritillaria Wuyangensis)by HPLC-ELSD.Methods The analysis was performed on a YMC-Triart C18 column(4.6 mm×250 mm,5μm)with a gradient of acetonitrile-0.1%TFA(v/v)aqueous solution at a flow rate of 1.0 mL·min^(-1) .The column temperature was set at 30℃.The detector was Alltech 2000 ES ELSD.The drift tube temperature was 105℃,and the carrier gas flow rate was 2.5 L·min^(-1) .Results Peimisine,verticine and verticinone were obtained with good linearity at 0.0409~0.4092,0.0499~0.4986,and 0.0421~0.4208 mg·mL^(-1) ,r≥0.9991.The recovery was 97.6%,96.3%,and 98.8%,respectively,RSD≤1.9%.Conclusion The method is simple,reproducible,accurate and reliable,which can be used for the simultaneous determination of 3 alkaloids in Fritillaria Wuyangensis.

关 键 词：舞阳贝母 高效液相色谱-蒸发光散射检测器 生物碱类成分 含量测定 

分 类 号：R917[医药卫生—药物分析学]