甘草总黄酮对高脂条件下罗非鱼肝损伤的保护作用  

Protective effects of Glycyrrhiza total flavones on liver injury of tilapia(Oreochromis niloticus)under high fat condition

作　　者：杜金梁[1,2,3] 曹丽萍[1,2] 贾睿[1,2] 顾郑琰 何勤 徐跑[1,2,4] JENEY Galina 马玉忠[3] 殷国俊[1,2,4] DU Jinliang;CAO Liping;JIA Rui;GU Zhengyan;HE Qin;XU Pao;JENEY Galina;MA Yuzhong;YIN Guojun(Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs/Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;International Joint Research Laboratory for Fish Immunopharmacology/Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China;Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;National Agricultural Research Center, Research Institute for Fisheries and Aquaculture, Szarvas 5440, Hungary)

机构地区：[1]中国水产科学研究院淡水渔业研究中心/农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心/鱼类免疫药理学国际联合实验室,江苏无锡214081 [3]河北农业大学动物医学院,河北保定071001 [4]南京农业大学无锡渔业学院,江苏无锡214081 [5]匈牙利水产与渔业研究所国家农业研究中心,匈牙利索尔沃什5440

出　　处：《浙江农业学报》2021年第10期1826-1835,共10页Acta Agriculturae Zhejiangensis

基　　金：中央级公益性科研院所基本科研业务费专项(2019JBFM10);江苏省自然科学基金(BK20201143)。

摘　　要：为了研究甘草总黄酮对高脂饲料造成的罗非鱼(Oreochromis niloticus)脂肪肝损伤是否具有保护作用,随机将180尾健康无伤罗非鱼分成6组,即对照组、高脂饲料模型组和4个不同浓度甘草总黄酮组(0.05、0.10、0.50、1.00 g·kg^(-1)甘草总黄酮),每组30尾。饲喂90 d后,采集罗非鱼各实验组肝,测定肝匀浆中超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)活性、丙二醛(MDA)含量、甘油三酯(TG)含量、总胆固醇(TC)含量、总抗氧化能力(T-AOC)水平,Western blotting法检测肝中核转录因子-κB P65(NF-κB P65)和核转录因子-κB C-Rel(NF-κB C-Rel)蛋白表达水平,RT-PCR法测定肝中微粒体甘油三酸酯转移蛋白(MTTP)和载脂蛋白B100(ApoB100)基因表达情况。结果表明:与对照组相比,高脂饲料模型组罗非鱼肝匀浆中TG、TC、MDA含量极显著(P<0.01)升高,GSH活性、SOD活性、T-AOC水平均极显著(P<0.01)降低;MTTP和ApoB100 mRNA表达水平降低(P<0.01或P<0.05);Western blotting结果显示,NF-κB P 65和C-Rel蛋白表达水平升高。饲料中加入甘草总黄酮后,与高脂饲料模型组相比,罗非鱼肝中GSH活性、SOD活性、T-AOC水平不同程度提高,TC、TG、MDA含量和NF-κB P65、C-Rel蛋白表达水平不同程度降低,MTTP和ApoB100的mRNA表达水平升高,以0.50 g·kg^(-1)甘草总黄酮组效果较好。说明甘草总黄酮对于罗非鱼脂肪肝损伤具有一定的保护作用,可以缓解高脂饲料造成的脂肪肝损伤。In order to evaluate protective effects of Glycyrrhiza total flavones(GTF)on liver of tilapia(Oreochromis niloticus)after fatty liver injury,180 healthy and uninjured tilapia were randomly divided into six experimental groups.Namely,control group(CK),high-fat diet(HFD)model group and four different concentrations(0.05,0.10,0.50,1.00 g·kg^(-1))of Glycyrrhiza total flavones treatment groups,each group 30 tails.After 90 days of feeding,liver were collected for determination activities of superoxide dismutase(SOD),reduced glutathione(GSH),contents of malondialdehyde(MDA),triglyceride(TG)and total cholesterol(TC),and level of total antioxidant capacity(T-AOC)in liver homogenate.Western blotting method was used to measure the protein levels of nuclear transcription factor-κB C-rel(NF-κB C-Rel)and nuclear transcription factor-κB P 65(NF-κB P65)in liver.Quantitative real-time PCR method was used to measure mRNA levels of microsomal triglyceride transfer protein(MTTP)and apolipoprotein B100(ApoB100).The results showed that:compared with the control group,contents of TG,TC and MDA in tilapia liver homogenate in high-fat diet group significantly increased(P<0.01),activities of GSH,SOD and T-AOC level in liver homogenate significantly decreased(P<0.01),and mRNA levels of MTTP,ApoB100 down-regulated(P<0.01 or P<0.05),NF-κB P65 and C-rel protein levels increased.After adding Glycyrrhiza total flavones to the diet,activities of GSH,SOD and T-AOC level in liver were increased in different degrees,contents of TC,TG,MDA,and NF-κB C-Rel,NF-κB P65 protein levels were decreased in different degrees,mRNA levels of MTTP and ApoB100 showed an up-regulated trend,and concentration of Glycyrrhiza total flavones at 0.50 g·kg^(-1) showed the best effects.Overall,these results indicated that Glycyrrhiza total flavones had certain effects on tilapia fatty liver and could alleviate the fatty liver injury caused by high-fat diet.

关 键 词：甘草 总黄酮 罗非鱼 脂肪肝 基因表达 Western blotting 

分 类 号：S965.125[农业科学—水产养殖]