物理和化学处理在翅果油树种子萌发中对酶活性的影响  

Effects of Physical and Chemical Interventions for Enzyme Activities during Seed Germination of Elaeagnus mollis Diels

作　　者：翟静娟[1] ZHAI Jingjuan(Shuozhou Vocational Technology College,Shuozhou,Shanxi 036002,China)

机构地区：[1]朔州职业技术学院,山西朔州036002

出　　处：《天津农业科学》2021年第10期8-12,共5页Tianjin Agricultural Sciences

基　　金：山西省化学优势重点学科建设项目(912019);山西省留学基金项目(20081073)。

摘　　要：为探讨不同物理和化学处理对翅果油树种子萌发的影响,本研究对翅果油树种子分别给予变温、激光、超声波、H2O2和PEG处理,以无处理种子作为对照,测定种子萌发过程(3,6,9,12,15,18 d)中的相关酶活性以及丙二醛含量的变化。结果表明:不同处理对翅果油树种子萌发过程中酶活性和丙二醛含量的影响存在差异,主要表现在相关指标随着萌发时间的波动变化方面。其中,超氧化物歧化酶活性变异系数表现为激光>变温>H2O2>超声波>对照>PEG;过氧化物酶活性变异系数表现为H2O2>激光>超声波>变温>对照>PEG;过氧化氢酶活性变异系数表现为超声波>H2O2>激光>PEG>变温>对照;抗坏血酸过氧化物酶活性变异系数表现为变温>H2O2>超声波>对照>激光>PEG;异柠檬酸裂解酶活性变异系数表现为对照>激光>超声波>PEG>H2O2>变温;MDA含量变异系数表现为激光>变温>超声波>PEG>H2O2>对照。综上,不同物理和化学处理会影响翅果油树种子萌发过程中相关酶活性及丙二醛含量的变化,但其与翅果油树种子萌发的关系仍需要进一步研究。To analyze the effects of different physical and chemical interventions during the seed germination of Elaeagnus mollis Diels,seeds of Elaeagnus mollis Diels were dealt with variable temperature,laser,ultrasonic,H2O2,and PEG respectively,no interventions seeds were used for as control.The related enzymes activities and malonaldehyde content were measured during the seed germination(3,6,9,12,15,18 d).The results showed that the effects on enzyme activity and malondialdehyde content were various among different interventions during the seed germination of Elaeagnus mollis Diels,which mainly involved the fluctuation of related indexes following with the germination time.The variable coefficient of superoxide dismutase was manifested as laser>variable temperature>H2O2>ultrasonic>control>PEG;variable coefficient of peroxidase was manifested as H2O2>laser>ultrasonic>variable temperature>control>PEG;variable coefficient of catalase was manifested as ultrasonic>H2O2>laser>PEG>variable temperature>control;variable coefficient of ascorbate peroxidase was manifested as variable temperature>H2O2>ultrasonic>control>laser>PEG;variable coefficient of isocitrate lyase was manifested as control>laser>ultrasonic>PEG>H2O2>variable temperature;variable coefficient of MDA was manifested as laser>variable temperature>ultrasonic>PEG>H2O2>control.To sum up,different physical and chemical interventions may impact the related enzymes activities and malonaldehyde content during the seed germination of Elaeagnus mollis Diels,but further studies are needed to investigate the relationship between these interventions and seed germination of Elaeagnus mollis Diels.

关 键 词：翅果油树 种子 萌发 酶活性 

分 类 号：Q945.34[生物学—植物学]