基于SWChen系统预测靶向林麝(Moschusberezovskii)FASN、HMGCR和CYP11A1基因miRNAs的研究  

Predicting miRNA Targeting FASN,HMGCR and CYP11A1 Genes in Moschus berezovskii Based on SWChen System

作　　者：谌颖莲 曾德军[1] 赵贵军[1] 封孝兰[1] 张承露[1] 吴佳勇 竭航[1] CHEN Yinglian;ZENG Dejun;ZHAO Guijun;FENG Xiaolan;ZHANG Chenglu;WU Jiayong;JIE Hang(Key Laboratory of Sichuan and Chongqing Co-construction for Characteristic Biological Resources Research and Utilization,Chongqing Institute of Drug Cultivation,Chongqing 408435,China)

机构地区：[1]重庆市药物种植研究所,特色生物资源研究与利用川渝共建重点实验室,重庆408435

出　　处：《云南农业大学学报（自然科学版）》2021年第5期880-890,共11页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(81973428);重庆市基本科研业务费项目(cstc2020-jbky001);重庆市自然科学基金项目(cstc2019jcyj-msxmX0024)。

摘　　要：【目的】预测靶向林麝(Moschus berezovskii)FASN、HMGCR和CYP11A1基因的miRNAs,为麝香的生物合成机制研究提供参考。【方法】通过林麝麝香的代谢组学测序,获取麝香中的主要化学组分,确定相应化学组分合成的关键酶;通过转录组测序获取林麝基因mRNA序列及miRNAs序列,利用自主编写的离线SWChen系统对调控关键酶基因表达的miRNA进行预测,并以人FSAN基因对系统准确性进行验证,同时以牛(Bos taurus)、山羊(Capra hircus)和绵羊(Ovis aries)预测结果进行比较和集合论分析,获取调控林麝基因的候选miRNAs。【结果】麝香的化学成分主要包括脂肪酸碳链类衍生物、芳香族、固醇类和甾类等衍生物质;预测调控FASN基因表达的miRNA主要为miR-24-3p和miR-30b-3p,调控HMGCR基因表达的为miR-29b、miR-146a、miR-181b-3p和miR-429家族,调控CYP11A1基因表达的为miR-532-3p和let-7家族,共同调控3个基因表达的主要为miR-205家族和miR-143家族。【结论】上述miRNAs为靶向调控林麝FASN、HMGCR和CYP11A1基因表达的候选miRNAs,对解析林麝麝香的生物合成机制具有参考价值。[Purpose]To predict the miRNAs targeting FASN,HMGCR and CYP11A1 genes in Moschus berezovskii for providing the reference about mechanism of musk biosynthesis.[Method]We acquired chemical components of musk to seek out key enzymes by metabolomics sequencing.Then,sequences of FASN,HMGCR and CYP11A1 mRNA sequences as well as miRNA sequences data of M.berezovskii were obtained by transcriptomic sequencing of musk gland.SWChen system written by ourselves had been used to screen candidate miRNAs of pertinent genes and had been verified the veracity.Meanwhile,the predicted results of Bos taurus,Capra hircus and Ovis aries species were used to compare to get candicate miRNAs regulating genes of M.berezovskii.[Result]The chemical substances of M.berezovskii mainly included in carbon chain fatty acid derivatives,aromatic,sterols and steroid derivative matters.The predicted results revealed that the miRNAs regulating FASN gene expression of M.berezovskii mainly constituted as miR-24-3 p and miR-30b-3p.The predicted miR-29b,miR-146a,miR-181b-3p and miR-429 family preferentially had influence on HMGCR gene expression.Then,miR-532-3p and let-7 family had been displayed as the modulation to CYP11A1 gene expression.Further,the miR-205 family and miR-143 family could jointly regulate the expression of the three genes.[Conclusion]The above predicted miRNAs are candidate regulators targeting the expression of FASN,HMGCR and CYP11A1 in M.berezovskii,which had the referential value for the analysis of the biosynthesis mechanism of musk in M.berezovskii.

关 键 词：林麝 FASN HMGCR CYP11A1 MIRNA 

分 类 号：S865.411[农业科学—野生动物驯养]