贵阳某野生动物园芽囊原虫感染调查及基因分型  

Investigation of Infection and Genotyping of Blastocystis sp.in a Wildlife Park in Guiyang

作　　者：陈礼颖 柴宜均 姚静新 陈善瑜 周紫峣[1] 刘海峰 钟志军[1] 彭广能[1] CHEN Liying;CHAI Yijun;YAO Jingxin;CHEN Shanyu;ZHOU Ziyao;LIU Haifeng;ZHONG Zhijun;PENG Guangneng(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学动物医学院,四川成都611130

出　　处：《云南农业大学学报（自然科学版）》2021年第5期925-929,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：四川卧龙国家级自然保护区管理局林业改革发展专项资金(510000-02-064387);成都大熊猫繁育研究基地项目(CPF2017-12)。

摘　　要：【目的】了解贵阳某野生动物园的圈养野生动物芽囊原虫的感染情况及是否存在人畜共患风险。【方法】对从贵阳某野生动物园采集的203份粪样收集芽囊原虫卵囊,采用PCR方法扩增SSU rRNA基因序列,并进行系统发育分析。利用ClustalX 2.0程序,根据Sanger测序结果确定芽囊原虫的亚型。【结果】203份样品中芽囊原虫阳性粪便共22份,总感染率为10.84%。鉴定出4个人兽共患亚型(ST1、ST2、ST3和ST8)和1个动物特异亚型(ST13),其中ST1(36.36%)为优势亚型。此外,本研究在国内首次报道黑猴存在芽囊原虫感染(1/7)。【结论】贵阳某野生动物园的野生动物不仅存在芽囊原虫感染,而且具有潜在的传染人的风险。研究结果将加深人们对芽囊原虫遗传多样性和公共卫生潜力的理解。[Purpose]To know about the infection and zoonotic risk of Blastocystis sp.in captive wild animals from a wildlife park in Guiyang.[Method]203 fecal specimens collected from the park were screened for Blastocystis sp.oocysts.The samples were subjected to PCR amplification of SSU rRNA gene and phylogenetic analysis.The ClustalX 2.0 program was used to determine the subtypes of Blastocystis based on the results of Sanger sequencing.[Results]There were 22 Blastocystispositive feces,and the total infection rate was 10.84%among the 203 samples.Four zoonotic subtypes(ST1,ST2,ST3,ST8)and one animal-specific subtype(ST13)were identified,among which ST1(36.36%)was the dominant.In addition,Macaca nigra was found for the first time to be infected with Blastocystis sp.(1/7)in China.[Conclusion]Captive wild animals from the wildlife park had not only Blastocystis infection,but also potential zoonotic risks.The results of this study will deepen our understanding of the genetic diversity and public health potential of Blastocystis.

关 键 词：芽囊原虫 野生动物 ST亚型 人畜共患风险 

分 类 号：S855.99[农业科学—临床兽医学]