跑轮运动对小鼠皮质神经元AKT/mTOR通路活性及运动诱发电位的影响  

Effects of wheeling-running exercise on AKT/mTOR pathway activity and motor-evoked potential in cortical neurons of mice

作　　者：易凌荣 谭波涛[1] 詹祖雄 刘媛 殷樱[1] 虞乐华[1] YI Ling-rong;TAN Bo-tao;ZHAN Zu-xiong;LIU Yuan;YIN Ying;YU Le-hua(Department of Rehabilitation Medicine,The Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;State Key Laboratory of Trauma,Burns and Combined Injury,Daping Hospital,Research Institute of Surgery,Army Medical University,Chongqing 400042,China)

机构地区：[1]重庆医科大学附属第二医院康复医学科,重庆400010 [2]陆军军医大学大坪医院野战外科研究部,创伤、烧伤与复合伤国家重点实验室,重庆400042

出　　处：《上海交通大学学报（医学版）》2021年第10期1285-1289,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(81702221,82002377);重庆市自然科学基金(cstc2018jcyjAX0180,cstc2019jcyj-msxmX0195,cstc2020jcyj-msxm0651);重庆市渝中区基础研究与前沿探索项目(20180121)。

摘　　要：目的·观察不同强度的跑轮运动对小鼠皮质神经元蛋白激酶B/哺乳动物雷帕霉素靶蛋白(protein kinase B/mammalian target of rapamycin,PKB/mTOR,AKT/mTOR)通路的激活情况及对神经传导的影响。方法·选取24只成年C57BL/6J小鼠为研究对象,随机分为对照组、低运动强度(low exercise intensity,LEI)组、中等运动强度(moderate exercise intensity,MEI)组和高运动强度(high exercise intensity,HEI)组,每组6只。4组小鼠接受为期3 d的预训练,结束后分别行不同强度的正式训练(1周)。检测运动诱发电位(motor-evoked potential,MEP)观察小鼠右上肢的神经传导情况。采用蛋白质印迹(Western blotting)检测小鼠运动皮质中AKT、核糖体蛋白S6(ribosomal S6 protein,S6)、胰岛素样生长因子1(insulin-like growth factor 1,IGF1)和脑源性生长因子(brain-derived neurotrophic factor,BDNF)表达情况。结果·MEP检测显示,与对照组相比,LEI组、MEI组和HEI组小鼠MEP的N1潜伏期有所缩短(均P<0.05);与HEI组相比,LEI组小鼠的N1潜伏期更短(P=0.032)。同时,与对照组相比,LEI组小鼠MEP的P1潜伏期亦有缩短(P=0.015)。Western blotting结果显示,与对照组相比,LEI组、MEI组和HEI组小鼠运动皮质中p-AKT/AKT和p-S6/S6的表达水平明显升高(均P<0.05);LEI组小鼠运动皮质中IGF1、BDNF的表达水平较对照组有所增加(均P<0.05)。结论·低强度运动训练能有效激活皮质神经元AKT/mTOR通路,提高神经传导速度,这可能与升高的神经生长因子水平相关。Objective·To investigate the effects of various wheel-running exercise intensity on activation of protein kinase B/mammalian target of rapamycin(PKB/mTOR,AKT/mTOR)pathway in cortical neurons and nerve conduction in mice.Methods·Twenty four adult C57BL/6J mice were randomly divided into control group,low exercise intensity(LEI)group,moderate exercise intensity(MEI)group and high exercise intensity(HEI)group,with 6 mice in each group.The four groups of mice received pre-training for 3 days,and then received formal training(1 week)with different intensity.Motor-evoked potential(MEP)was used to detect the nerve conduction in the right upper limb of mice.The expression of AKT,ribosomal S6 protein(S6),insulin-like growth factor 1(IGF1)and brain-derived growth factor(BDNF)in the motor cortex of mice were detected by Western blotting.Results·MEP test showed that compared with the control group,the N1 latency period of MEP in the LEI group,MEI Group and HEI group was shorter(all P<0.05);compared with the HEI group,the N1 latency period in the LEI group was shorter(P=0.032).Meanwhile,compared with the control group,the P1 latency period of MEP in the LEI group was also shorter(P=0.015).Western blotting results showed that compared with the control group,the expression levels of p-AKT/AKT and p-S6/S6 in the motor cortex of mice in the LEI group,MEI Group and HEI group were significantly increased(all P<0.05);the expression levels of IGF1 and BDNF in the motor cortex of mice in the LEI group were higher than those in the control group(both P<0.05).Conclusion·Low intensity exercise training can activate AKT/mTOR pathway of cortical neurons and improve the nerve conduction speed,which may be related to the increased nerve growth factors.

关 键 词：训练强度 蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号通路 神经传导 生长因子 皮质神经元 

分 类 号：R493[医药卫生—康复医学]