用9H-吡啶(3,4-b)吲哚制备钝顶螺旋藻原生质球  

Preparation of protoplast from Spirulina platensis by using 9H-pyridine(3,4-b)indole

作　　者：张小利 朱昌宇 宫利敏 ZHANG Xiaoli;ZHU Changyu;GONG Limin(Life Science and Technology School, Inner Mongolia University of Science and technology, Baotou 014010, China)

机构地区：[1]内蒙古科技大学生命科学与技术学院,内蒙古包头014010

出　　处：《内蒙古科技大学学报》2021年第3期205-208,共4页Journal of Inner Mongolia University of Science and Technology

基　　金：内蒙古自治区自然科学基金资助项目(2017MS0336);内蒙古科技大学大学生科技与创新基金资助项目(2015014).

摘　　要：用溶藻菌分泌有效物质9H-吡啶(3,4-b)吲哚制备钝顶螺旋藻原生质球.钝顶螺旋藻培养液经超离心分离后,用磷酸盐缓冲液稀释藻体至A560在0.6~0.8范围内,再进行破壁处理.最后结果表明在50 mg/L 9H-吡啶(3,4-b)吲哚溶液,渗透稳定剂KCl浓度为1.7 mol/L,pH值为6.0,于30℃下恒温水浴震荡12 h能得到最佳的原生质球得率.The protoplast of Spirulina platensis was prepared by the release of 9H-pyridine(3h-4-b)indoles from algicidal bacteria.The culture medium of Spirulina platensis was separated by ultracentrifugation,and then diluted with phosphate buffer to A560 in the range of 0.6~0.8.The results show that the optimal protoplast yield can be obtained in the solution of 9H-pyridine(3h-4-b)indoles at a concentration of 50 mg/L,when the concentration of osmotic stabilizer KCl is 1.7 mol/L,and pH=6.0 at 30℃under constant temperature water bath for 12 h.

关 键 词：原生质球 9H-吡啶(3 4-b)吲哚 钝顶螺旋藻 溶藻菌 

分 类 号：Q28[生物学—细胞生物学]