基于TLR4/NF-κB通路研究益气活血药对心梗大鼠心室功能的影响  

Effects of Yiqi Huoxue Decoction on ventricular function in rats with myocardial infarction based on TLR4/NF-κB pathway

作　　者：冯鹏飞 郭书文[2] 魏路路 王惠 刘云柯 魏梵域 FENG Pengfei;GUO Shuwen;WEI Lulu;WANG Hui;LIU Yunke;WEI Fanyu(College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中医学院,100029 [2]北京中医药大学房山医院心内科

出　　处：《环球中医药》2021年第10期1761-1766,共6页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81774031);北京市自然科学基金(7202119)

摘　　要：目的观察益气活血药对心梗大鼠心室功能的影响并探讨其作用机制。方法构建大鼠心梗模型(结扎左前降支),随机分组模型组、培哚普利组、益气活血组,每组8只,另假手术8只作为对照;给药28天超声心动图检测各组大鼠左室功能变化;苏木精—伊红染色(hematoxylin-eosin staining,HE)观察各组大鼠心肌组织病理形态;免疫印迹法(Western blot,WB)检测梗死边缘区心肌组织Toll样受体4(toll-like receptor 4,TLR4)、核因子κB(nuclear factor kappa-B,NF-κB)蛋白表达水平;酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)检测血清组织中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、单核细胞趋化蛋白1(monocyte chemotactic protein 1,MCP-1)及白细胞介素-6(interleukin-6,IL-6)含量。结果(1)小动物心脏超声心动图显示:较假手术组,模型组左室舒张末期内径(left ventricular end diastolic diameter,LVIDd)、左室收缩末期内径(leftventricular internal dimension systole,LVIDs)均显著升高(P<0.05),左室射血分数(left ventricularejection fraction,LVEF)、左室短轴率(left ventricular fractional shortening,LVFS)均显著降低(P<0.05);较模型组,两给药组LVIDs显著性降低(P<0.05)、LVEF、LVFS均显著性升高(P<0.05)、LVIDd无明显变化(P>0.05);各给药组相比,LVIDd、LVIDs、LVEF、LVFS,各指标变化均无统计学差异。(2)WB结果显示:较假手术组,模型组TLR4、NF-κB蛋白表达显著性升高(P<0.05);较模型组,两给药组TLR4、NF-κB蛋白表达显著性降低(P<0.05);用药组间,TLR4、NF-κB蛋白表达无统计学差异。(3)ELISA结果显示:较假手术组,模型组血清TNF-α、MCP-1及IL-6的含量显著性升高(P<0.05);较模型组,两给药组血清TNF-α、MCP-1及IL-6的含量显著性降低(P<0.05);各给药组相比,血清TNF-α、MCP-1及IL-6的含量变化无统计学差异。结论益气活血药对心梗大鼠心室功能具有保护作用,可能通过TLR4/NF-κB信号通路抑制慢性炎症反应Objective To observe the effect of Yiqi Huoxue Decoction on ventricular function and investigate its mechanism.Methods The rats were randomly divided into model group,perindopril group,Yiqi Huoxue group,8 rats in each group,and 8 rats in sham operation group as control group.The changes of left ventricular function were detected by echocardiography 28 days after administration;the pathological morphology of myocardial tissue was observed by hematoxylin eosin staining(HE);Western blot(WB)was used to detect the protein expression of Toll like receptor 4(TLR4)and nuclear factor kappa-B(NF-κB)in myocardial tissue of infarct border area;enzyme linked immunosorbent assay(ELISA)was used to detect tumor necrosis factorα(TNF-α)in serum The levels of TNF-α,MCP-1 and IL-6 were measured.Results(1)Echocardiography of small animals showed that compared with the sham operation group,the left ventricular end diastolic diameter(LVIDd),left ventricular internal dimension systole(LVIDs)in the model group were significantly increased(P<0.05),left ventricular ejection fraction(LVEF)and left ventricular fractional velocity(LVEF)Compared with the model group,LVIDs of the two groups were significantly decreased(P<0.05),LVEF and LVFS were significantly increased(P<0.05),LVIDd had no significant change;compared with the model group,LVIDd,LVIDs,LVEF and LVFS of the two groups had no significant difference(P>0.05).(2)WB results showed that:compared with the sham operation group,the expression of TLR4 and NF-κB protein in the model group was significantly increased(P<0.05);compared with the model group,the expression of TLR4 and NF-κB protein in the two treatment groups was significantly decreased(P<0.05);there was no significant difference in the expression of TLR4 and NF-κB protein between the treatment groups.(3)ELISA results showed that:compared with the sham operation group,the levels of serum TNF-α,MCP-1 and IL-6 in the model group were significantly increased(P<0.05);compared with the model group,the levels of serum TNF-α,MCP

关 键 词：心肌梗死 益气活血药 心室功能 Toll样受体4 核因子ΚB 炎症反应 

分 类 号：R285.5[医药卫生—中药学]