环介导等温扩增技术研究进展  

Research progress of loop-mediated isothermal amplification technology

作　　者：石磊[1] 王曼 时国强 董振国 刘昱 SHI Lei;WANG Man;SHI Guoqiang;DONG Zhenguo;LIU Yu(Institute of Food Safety and Nutrition,Jinan University,Guangzhou 510000,China;College of Life Science,Agricultural University of Hebei,Baoding 071003,China;Research and Development Department,Hebei Sanshi Biological Technology Co.,L.TD,Shijiazhuang 050000,China)

机构地区：[1]暨南大学食品安全与营养研究院,广东广州510000 [2]河北农业大学生命科学学院,河北保定071003 [3]河北三狮生物科技有限公司研发部,河北石家庄050000

出　　处：《河北大学学报（自然科学版）》2021年第5期565-571,共7页Journal of Hebei University(Natural Science Edition)

基　　金：河北省创新能力提升计划项目(205A7601D);石家庄市高层次科技创新创业人才项目(05202001)。

摘　　要：环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术是一种高效、简便、高特异性、无需热循环设备的核酸扩增技术.由于LAMP依然具备巨大的改造潜力,所以近些年来,一直有研究改进其技术以期达到更好的扩增检测效果.本文对LAMP的基本原理、应用领域范围以及技术改良方向进行了综述,为今后该技术的改良与发展提供参考.Loop-mediated isothermal amplification(LAMP)is an efficient,simple,highly specific nucleic acid amplification technology without thermal cycling equipment.As LAMP still has great potential for modification,many studies have been conducted in recent years to improve its technology in order to achieve better amplification detection results.In this paper,the basic principle,application scope and technical improvement direction of LAMP were reviewed,to provide references for the improvement and development of LAMP technology in the future.

关 键 词：环介导等温扩增(LAMP) 技术改良 发展方向 

分 类 号：Q7[生物学—分子生物学]