响应面优化提取美藤果油及其对高强度运动大鼠腓肠肌功能恢复和ATP酶的影响  

Response Surface Methodology for Optimization of Rattan Oil Extraction and Effects of the Oil on the Functional Recovery of the Gastrocnemius and ATPase in Rats After High Intensity Exercise

作　　者：齐婷[1] 房磊[2] QI Ting;FANG Lei(Jilin Institute of Chemical Technology,Jilin 132022,Jilin,China;Jilin Agricultural Science and Technology University,Jilin 132101,Jilin,China)

机构地区：[1]吉林化工学院,吉林吉林132022 [2]吉林农业科技学院,吉林吉林132101

出　　处：《食品研究与开发》2021年第19期53-58,共6页Food Research and Development

基　　金：吉林省教育厅科学技术研究规划项目(JJKH20190977KJ)。

摘　　要：采用响应面优化超临界CO2萃取美藤果油工艺,并研究其对高强度运动下大鼠腓肠肌功能恢复和ATP酶的影响。结果表明:萃取美藤果油的最优工艺条件为萃取压力21 MPa、萃取温度55℃、萃取时间109 min,此条件下美藤果油的得率为50.15%。大鼠实验表明,灌胃美藤果油后,由于其具有较强的抗氧化能力,可以提高过氧化物酶(peroxidase,POD)活性和琥珀酸脱氢酶(succinate dehydrogenase,SDH)水平,减少乳酸的产生,提高三磷酸腺苷(adenosine triphosphate,ATP)酶合成能力,清除机体内产生的自由基,避免脂质过氧化,缓解运动性疲劳,进而使得大鼠腓肠肌功能恢复。Response surface methodology was used to optimize the supercritical CO2 extraction process of Rattan oil,and the effects of the oil on recovery of gastrocnemius muscle function and ATPase after high-intensity exercise were studied.The optimum extraction conditions were determined as extraction pressure of 21 MPa,extraction temperature of 55℃and extraction time of 109 min.Under these conditions,the yield of Rattan oil was 50.15%.The results showed that after intragastric administration of the oil,the activity of peroxidase and the level of succinate dehydrogenase increased,the production of lactic acid reduced,and the activity of adenosine triphosphate(ATP)increased.This was facilitated by strong antioxidant capacity and ATP synthesis ability,elimination of free radicals produced in the body,avoidance of lipid peroxidation,alleviation of exercise-induced fatigue,and recovery of gastrocnemius function.

关 键 词：美藤果油 超临界萃取 高强度运动 腓肠肌 ATP酶 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]