MIRA-LFD鉴别肉制品中鸭源性成分的 方法研究  

Detection of Duck-derived Production Using MIRA-LFD

作　　者：尚柯 张敏 张彪 王巍 段庆梓 张玉 雍溶 叶梅 SHANG Ke;ZHANG Min;ZHANG Biao;WANG Wei;DUAN Qing-zi;ZHANG Yu;YONG Rong;YE Mei(Chengdu Food and Drug Inspection Institute,Chengdu 611135,Sichuan,China;College of Biology,Hunan University,Changsha 410012,Hunan,China)

机构地区：[1]成都市食品药品检验研究院,四川成都611135 [2]湖南大学生物学院,湖南长沙410012

出　　处：《食品研究与开发》2021年第19期179-186,共8页Food Research and Development

基　　金：国家市场监督管理总局技术保障专项项目(2020YJ031)。

摘　　要：传统的肉制品动物源性成分检测对检测人员操作水平及试验条件等方面要求较高,不适于基层检测需要。多酶恒温快速扩增技术(multienzyme isothermal rapid amplification,MIRA)是一种新型的核酸扩增方式,整个反应在37℃条件下即可完成,相比昂贵的聚合酶链式反应(polymerase chain reaction,PCR)仪器,检测成本以及试验条件要求明显降低。该研究通过结合多酶恒温快速扩增技术与侧向流层析技术(lateral flow dipstick,LFD),建立一种鸭源性成分多酶恒温侧流式扩增技术(MIRA-LFD)的快速检测方法,并对其特异性、检出性以及应用的可行性进行评价。Traditional methods for detection of meat and meat products require relatively high levels of personnel operation and expensive experimental procedures;these requirements can′t be met in grass-roots testing.Multienzyme isothermal rapid amplification(MIRA)is a type of nucleic acid amplification whereby the entire reaction can be completed at 37℃.Compared with the polymerase chain reaction(PCR)instrument,detection and testing using MIRA are significantly less expensive.In addition,the detection criteria are considerably relaxed.In this study,a MIRA-lateral flow dipstick(LFD)method was developed for rapid and simple detection of duck-derived materials.Moreover,the specificity,detection limit and feasibility of on-site testing of duck-derived materials were evaluated through experiments.

关 键 词：物种鉴定 鸭源性成分 多酶恒温快速扩增技术 侧向流层析技术 方法考察 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]