鲜食甜、糯玉米自交系对拟轮枝镰孢菌穗粒腐病的抗性鉴定  

Evaluation of fresh maize inbred lines for resistance to Fusarium verticillioides ear rot

作　　者：胡颖雄 王慧[1,2,3] 靳林朋 关媛[1,2,3] 孙萍东 卫季辉 郑洪建[1,2,3] 林金元[1,2,3] HU Yingxiong;WANG Hui;JIN Linpeng;GUAN Yuan;SUN Pingdong;WEI Jihui;ZHENG Hongjian;LIN Jinyuan(Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;CIMMYT-China Specialty Maize Research Center,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Shanghai Engineering Research Center of Specialty Maize,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;College of Life Sciences,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海市农业科学院作物育种栽培研究所,上海201403 [2]上海市农业科学院CIMMYT-中国特用玉米研究中心,上海201403 [3]上海市农业科学院上海特用玉米工程技术研究中心,上海201403 [4]上海师范大学生命科学学院,上海200234

出　　处：《上海农业学报》2021年第5期12-19,共8页Acta Agriculturae Shanghai

基　　金：上海市科技兴农项目[沪农科创字(2018)第1-3号];上海市国际科技合作基金项目(19390743200);科技部重点研发计划(2018YFD0100106-3);一带一路中越农业科学国际联合实验室项目(20310750500)。

摘　　要：以形态学和分子生物学为基础,对鲜食甜、糯玉米穗粒腐病样品中致病菌拟轮枝镰孢菌进行分离鉴定。针对37份不同来源的鲜食甜、糯玉米自交系种质材料,采用针刺注射果穗接种法进行人工接种拟轮枝镰孢菌,并对其抗性进行鉴定。结果表明:不同鲜食玉米自交系种质的抗病性差异较大,Hw53、Hw71、Hw175、Ht219和Ht253共5份自交系对拟轮枝镰孢菌表现为高抗,而Hw61、Hw144、Hw145等10份自交系对该致病菌表现为高感。在22份糯玉米自交系种质中,对拟轮枝镰孢菌表现感病的种质有8份,占参试糯玉米自交系种质总数的36.36%;在15份甜玉米自交系种质中,对拟轮枝镰孢菌表现感病的种质有7份,占参试甜玉米自交系种质总数的46.67%,甜玉米相对糯玉米更易感染穗粒腐病。该研究结果可为鲜食甜、糯玉米穗粒腐病的综合绿色防治及抗性品种选育提供一定的理论依据。The Fusarium verticilloides was isolated and identified from fresh maize ear rot samples with morphological and molecular techniques.The artificial inoculation test was carried out on 37 inbred lines of fresh maize by needle injection and the resistance was identified.The results showed that different fresh maize inbred lines represented the variation of resistance.The 5 inbred lines,including Hw53,Hw71,Hw175,Ht219 and Ht253,showed highly resistant against F.verticilloides,while the 10 inbred lines,including Hw61,Hw144,Hw145,were highly susceptible.The 8 of 22 waxy maize inbred lines were susceptible to F.verticilloides,accounting for 36.36%of the total number of waxy maize inbred lines.The 7 of 15 sweet maize inbred lines were susceptible to F.verticilloides,accounting for 46.67%of the total number of sweet maize inbred lines.Sweet maize was more susceptible to ear rot than waxy maize.These results provided the theoretical basis for integrative disease control and breeding of fresh maize for resistance to ear rot.

关 键 词：鲜食玉米 穗粒腐病 拟轮枝镰孢菌 抗病性 

分 类 号：S513[农业科学—作物学]