基于SSR分子标记的栎属植物资源的亲缘关系分析  

Analysis of genetic relationship of Quercus germplasm resources based on SSR molecular markers

作　　者：关媛[1] 吕秀立 于泽群 李秀芬[1] 李水根[1] 刘群录 朱建军[1] GUAN Yuan;LÜXiuli;YU Zequn;LI Xiufen;LI Shuigen;LIU Qunlu;ZHU Jianjun(Forest and Fruit Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;Shanghai Academy of Landscape Architecture Science and Planning,Shanghai 200232,China;School of Design,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海市农业科学院林木果树研究所,上海201403 [2]上海市园林科学规划研究院,上海200232 [3]上海交通大学设计学院,上海200240

出　　处：《上海农业学报》2021年第5期20-25,共6页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目[沪农科攻字(2013)第5-10号]。

摘　　要：为了有效利用栎属植物资源,利用SSR标记技术对栎亚属4个树种24个单株进行亲缘关系分析。结果表明:从44对SSR引物中筛选出9对多态性引物,PCR结果显示,这9对引物在24份材料中共检测到62个等位基因变异,每对引物检测到的等位基因变异范围为4—12个,平均每对引物检测到6.8个等位基因变异。各位点的平均有效等位基因数为4.9633,Nei多样性指数分布范围在0.6285—0.8915,平均值为0.7586,Shannon信息指数为1.1843—2.3367。UPGMA法聚类分析结果显示,24份种质资源间遗传相似系数的平均值为0.7008,变化范围为0.4928—0.9710。栎亚属4个树种分为两大类,中国江苏收集的麻栎单独属于一大类,美国收集的3种树种属于一大类,其中落叶型的沼生栎和常绿型的弗吉尼亚栎为一类,常绿型的墨西哥白橡单独属于一类。研究可为栎属植物引种资源的管理及评价提供重要依据。In order to make effective use of the Quercus resources,24 individuals of four Quercus species were analyzed by SSR marker,and 9 pairs of polymorphic primers were selected from 44 SSR primers for amplification.A total of 62 alleles were detected in 24 materials,ranging from 4 to 12,with an average of 6.8 alleles per pair of primers.The average value of effective allelic variation of each site was 4.9633.The Nei diversity index ranged from 0.6285 to 0.8915,with an average of 0.7586.The Shannon’s information index ranged from 1.1843 to 2.3367.The DNA fingerprints of the 24 individuals of the 4 tree species were obtained by UPGMA method based on the genetic similarity coefficient matrix among individuals,the results indicated that the average genetic similarity coefficient among 24 germplasm resources was 0.7008,and the variation ranged from 0.4928 to 0.9710.The clustering results showed that all the resources were divided into two categories,the oak trees collected from Jiangsu,China belonged to a category,and the three species collected from the United States of America belonged to a category.Among them,deciduous marsh oak and evergreen Virginia Quercus were clustered into a class,while the evergreen Mexican white oak was clustered into a class.The results can provide important basis for the management and evaluation of introduced Quercus resources.

关 键 词：栎属植物 SSR标记 亲缘关系分析 

分 类 号：S792.18[农业科学—林木遗传育种]