新冠肺炎康复者恢复期血浆HLA抗体和MICA抗体检测及其分析  

Detection of anti-HLA and anti-MICA in convalescent plasma from individuals recovered from COVID-19

作　　者：马开荣[1] 张兵 陈琰 刘瑛[1] 许先国[1] 何吉[1] 朱发明[1] MA Kairong;ZHANG Binbing;CHEN Yan;LIU Ying;XU Xianguo;HE Ji;ZHU Faming(Blood Center of Zhejiang Province,Hangzhou 310052,China)

机构地区：[1]浙江省血液中心,浙江杭州310052

出　　处：《中国输血杂志》2021年第9期958-960,共3页Chinese Journal of Blood Transfusion

基　　金：国家卫生健康委科研基金(WKJ-ZJ-2021);浙江省科技计划项目(LGF19H080004);浙江省卫生创新人才项目。

摘　　要：目的分析新冠肺炎康复者恢复期血浆中人类白细胞抗原(HLA)抗体以及MHCⅠ类链相关基因A(MICA)抗体的阳性率情况。方法采用Luminex平台技术同步筛查HLA-Ⅰ、Ⅱ类抗体和MICA抗体。利用单抗原特异性试剂鉴定HLA-Ⅰ类抗体和Ⅱ类抗体特异性。不同组抗体筛查阳性率比较采用卡方检验。结果共收集到新冠肺炎康复者标本88份,HLA-Ⅰ类抗体、Ⅱ类抗体和MICA抗体阳性率分别为18.19%、19.32%、10.23%;HLA-Ⅰ类抗体、Ⅱ类抗体均阴性为64份(72.73%)。对照组随机献血者标本为95例,HLA-Ⅰ类抗体、Ⅱ类抗体和MICA抗体阳性率分别为8.42%、13.68%、10.53%;HLA-Ⅰ类抗体、Ⅱ类抗体均阴性为76份(80.00%)。2组间HLA-Ⅰ类抗体、Ⅱ类抗体以及MICA抗体阳性率差异均无统计学意义。每例新冠肺炎康复者恢复期血浆筛查阳性标本HLA抗体特异性针对的抗原表位不同,大多数抗体针对多个等位基因的抗原表位。结论新冠肺炎康复者恢复期血浆中存在一定比例的HLA抗体,筛选HLA抗体有助于提高输注安全性。Objective To analyze the positive rate of antibodies against human leukocyte antigen(HLA)and MHC class I chain-related gene A(MICA) in the convalescent plasma from individuals recovered from COVID-19. Methods HLA-Ⅰ,-Ⅱ and MICA antibodies were screened simultaneously by Luminex platform. The specificity of HLA-Ⅰ and-Ⅱ antibodies was identified by single antigen reagents.The positive rate of antibody in different groups were compared by Chi-square test.Results A total of 88 cases of convalescent plasma were collected, among which the positive rates of HLA-Ⅰ,-Ⅱ and MICA antibodies were 18.19%, 19.32% and 10.23%, respectively, and 64 individuals(72.73%) were negative for HLA-Ⅰ and-Ⅱ antibodies. 95 blood donors were randomly selected as the control group, and the positive rate of HLA-Ⅰ,-Ⅱ and MICA antibodies were 8.42%, 13.68% and 10.53%, respectively, and 76 individuals(80.00%) were negative for HLA-Ⅰ and-Ⅱ antibodies. There were no significant difference in the positive rates of HLA-Ⅰ,-Ⅱ and MICA antibodies between convalescent individuals and control group. The specificity of HLA antibody to epitopes was different in each convalescent individual with positive HLA antibodies, and most antibodies were targeted to the epitopes of multiple HLA alleles. Conclusion A certain proportion of HLA antibody was found in the convalescent plasma of individuals recovered from COVID-19. Therefore, HLA antibody screening is helpful to improve the safety of transfusion.

关 键 词：新冠肺炎 恢复期血浆 HLA抗体 MICA抗体 阳性率 

分 类 号：R457.11[医药卫生—治疗学]