海南黎族人群13个稀有血型系统等位基因多态性的研究  

Allelic polymorphism of 13 rare blood group systems in Li Ethnic Group in Hainan

作　　者：符小玲 蔡兴权[2] 杨时平[2] 赵兴丹 翁艾罕 夏兰[2] FU Xiaoling;CAI Xingquan;YANG Shiping;ZHAO Xingdan;WENG Aihan;XIA Lan(Department of Blood Transfusion,Hainan Women and Childrens Medical Center,Haikou 570311,China;Hainan General Hospital)

机构地区：[1]海南省妇女儿童医学中心输血科,海南海口570311 [2]海南省人民医院

出　　处：《中国输血杂志》2021年第9期970-974,共5页Chinese Journal of Blood Transfusion

基　　金：海南省自然科学青年基金项目(820QN410);海南省医药卫生科研项目(20A200453)。

摘　　要：目的对海南黎族人群Duffy、Diego、Kidd、Dombrock、MNS、Lutheran、Kell、Colton、Scianna、Yt、Knops、Indian、Vel 13个红细胞稀有血型系统等位基因进行检测,了解海南黎族人群稀有血型等位基因的分布特点,为临床输血安全提供参考依据。方法采用聚合酶链式反应-序列特异性引物法(PCR-SSP)对300名海南黎族人群13个红细胞稀有血型系统抗原基因进行分型,并分析基因频率。结果海南黎族人群的Duffy、Diego、Kidd、Dombrock、MNS、Lutheran血型系统等位基因频率分别是:Duffy:Fya=0.9583、Fyb=0.0417;Diego:Dia=0.0667、Dib=0.9333;Kidd:Jka=0.4500、Jkb=0.5500;Dombrock:Doa=0.1017、Dob=0.8983;MNS:M=0.8183、N=0.1817、S=0.0283、s=0.9717、Mur+=0.5767;Lutheran:Aua=0.8350,Aub=0.1650。海南黎族人群的Kell、Colton、Scianna、Yt、Knops、Indian、Vel血型系统抗原基因不具有多态性,基因型分别是kk、CoaCoa、Sc1Sc1、YtaYta、KnaKna、InbInb、Vel+。结论海南黎族人群的Duffy、Diego、Kidd、Drombrock、MNS、Lutheran血型系统等位基因呈多态性分布;Kell、Colton、Scianna、Yt、Knops、Indian、Vel血型系统等位基因呈单态性分布。Objective To detect alleles of rare blood group systems of red blood cells(RBC)of Duffy,Diego,Kidd,Dombrock,MNS,Lutheran,Kell,Colton,Scianna,YT,Knops,Indian and Vel of Li Ethnic Group in Hainan,so as to investigate the distribution characteristics of rare blood group alleles and provide references for safe blood transfusion.Methods The genotypes of 13 rare blood group systems in 300 Li people in Hainan were analyzed by PCR-SSP,and the gene frequency was analyzed.Results The allele frequencies of Duffy,Diego,Kidd,Dombrock,MNS,and Lutheran blood group systems of 300 Li people were as follows:Duffy:Fya=0.9583,Fyb=0.0417;Diego:Dia=0.0667,Dib=0.9333;Kidd:Jka=0.4500,Jkb=0.5500;Dombrock:Doa=0.1017,Dob=0.8983;MNS:M=0.8183,N=0.1817,S=0.0283,s=0.9717,Mur+=0.5767;Lutheran:Aua=0.8350,Aub=0.1650.The antigen genes of Kell,Colton,Scianna,Yt,Knops,Indian,Vel blood group systems were not polymorphic,and the genetypes were kk,CoaCoa,Sc1Sc1,YtaYta,KnaKna,InbInb and Vel+,respectively.Conclusion The allele frequencies of Duffy,Diego,Kidd,Dombrock,MNS and Lutheran blood group systems of Li ethnic group in Hainan were polymorphic,while the allele frequencies of Kell,Scianna,Yt,Knops,Colton,Indian and Vel blood group systems were monomorphic.

关 键 词：稀有血型 基因多态性 基因频率 稀有血型数据库 黎族 

分 类 号：R457.11[医药卫生—治疗学]