西瓜藤镇痛活性部位筛选及作用机制研究  

Screening of the Analgesic Fractions from Citrullus Lanatus Vine and Its Mechanism

作　　者：龚小妹[1] 欧春丽 侯小利[1] 张文玉 陈硕 周小雷[1] 王硕[1] GONG Xiaomei;OU Chunli;HOU Xiaoli;ZHANG Wenyu;CHEN Shuo;ZHOU Xiaolei;WANG Shuo(Institute of Guangxi Medicinal Plant,National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Nanning 530021,China;Department of Traditional Chinese Medicine,People's Hospital of Lixia District of Jinan,Shandong Province,Jinan 250000,China)

机构地区：[1]广西药用植物研究所,西南濒危药材资源开发国家工程实验室,南宁530021 [2]山东省济南市历下区人民医院中药科,济南250000

出　　处：《医药导报》2021年第11期1494-1499,共6页Herald of Medicine

基　　金：广西科技厅自然基金项目(2019GXNSFBA245003);广西科技厅自然基金项目(2020GXNSFAA259008);广西研究生创新创业教育暨联合培养基地示范建设项目(20170703);广西研究生创新创业教育暨联合培养基地示范建设项目(20180606);广西科学研究与技术开发计划项目(桂科攻10124008-12)。

摘　　要：目的研究西瓜藤不同部位提取物抗炎镇痛作用及其机制。方法采用2.5%甲醛溶液致痛实验,筛选西瓜藤抗炎镇痛活性部位;采用酶联免疫吸附测定(ELISA)检测小鼠血清一氧化氮(NO)、前列腺素E_(2)(PGE_(2))、5-羟色胺(5-HT)、β-内啡肽(β-EP)、P物质和肾上腺素(NE)含量;采用反转录聚合酶链反应评估注射2.5%甲醛溶液前后不同时间点小鼠脑环氧合酶1,2(COX-1、COX-2)表达情况。结果不同剂量西瓜藤乙酸乙酯部位提取物均能显著降低2.5%甲醛溶液致痛小鼠Ⅰ相与Ⅱ相镇痛评分值(P<0.05);大剂量西瓜藤乙酸乙酯部位提取物可降低2.5%甲醛溶液致痛小鼠血清P物质含量(P<0.05);大剂量、中剂量西瓜藤乙酸乙酯部位提取物能显著升高2.5%甲醛溶液致痛小鼠血清β-EP含量(P<0.05),降低血清NO、PGE_(2)、5-HT、NE含量(P<0.05),抑制小鼠脑组织COX-1与COX-2表达。结论西瓜藤镇痛作用有效部位可能存在于乙酸乙酯部位,其镇痛作用通过降低血清PGE_(2)、5-HT、NO、NE和P物质含量,升高β-EP含量,抑制小鼠大脑内COX-1与COX-2表达来实现,即西瓜藤乙酸乙酯部位提取物通过中枢镇痛和外周镇痛发挥作用。Objective To study the anti-inflammatory and analgesic effects of different fractions of Citrullus lanatus vine extract and its mechanism.Methods The pain experiment caused by 2.5%formaldehyde solution was used to screen the anti-inflammatory and analgesic active parts of Citrullus lanatus vine.The contents of nitric oxide(NO),prostagland E_(2)(PGE_(2)),5-hydroxytryptamine(5-HT),β-endorphin(β-EP),substance P and epinephrine(NE)in serum of mice induced by 2.5%formaldehyde solution were determined by ELISA kits.Reverse transcription polymerase chain reaction(RT-PCR)was used to evaluate the levels of COX-1 and COX-2 mRNA in the brain of mice before and after injection of 2.5%formaldehyde solution.Results The ethyl acetate extract of Citrullus lanatus vine could significantly reduce phase I and phase II analgesia scores of paw pain induced by 2.5%formaldehyde solution(P<0.05).High doses of ethyl acetate extract of Citrullus lanatus vine could significantly decrease the serum substance P content of the pain in mice(P<0.05),High and medium doses of ethyl acetate extract of Citrullus lanatus vine could significantly increase the serumβ-EP content of the pain in mice(P<0.05),decrease the contents of NO,PGE_(2),5-HT and NE in serum(P<0.05),and inhibit the expression of COX-1 and COX-2 in mice brain tissue.Conclusion The effective analgesic fraction of Citrullus lanatus vine may exist in the ethyl acetate part.The analgesic effect of Citrullus lanatus vine may release by decreasing the contents of PGE_(2),5-HT,NO,NE and substance P in serum,increasing the content ofβ-EP,and inhibiting the expression of COX-1 and COX-2 in mice brain.In other words,the ethyl acetate part of Citrullus lanatus vine plays a role through central and peripheral analgesia.

关 键 词：西瓜藤乙酸乙酯部位提取物 镇痛 筛选 

分 类 号：R286[医药卫生—中药学] R95[医药卫生—中医学]