高效液相色谱法测定莪术油乳膏含量  

Determination of Main Active Components of Zedoary Turmeric Oil Cream by HPLC

作　　者：李文静[1] 刘军[1] 李宏铃[1] 包亚男[1] 朱红 洪博[1] LI Wenjing;LIU Jun;LI Hongling;BAO Yanan;ZHU Hong;HONG Bo(School of Pharmacy,Qiqihar Medical University,Qiqihar 161006,China;Qiqihar Tuberculosis Prevention and Control Hospital,Heilongjiang Province,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔医学院药学院,齐齐哈尔161006 [2]黑龙江省齐齐哈尔市结核病防治院,齐齐哈尔161006

出　　处：《医药导报》2021年第11期1577-1579,共3页Herald of Medicine

基　　金：黑龙江省省属本科高校基本科研业务费项目(2019-KYYWF-1244)。

摘　　要：目的采用高效液相色谱法建立莪术油乳膏含量测定方法。方法色谱柱采用安捷伦(Agilent)TC-C 18柱(4.6 mm×250 mm,5μm),流动相:乙腈-水溶液梯度洗脱,检测波长:216 nm,柱温25℃,流速:1.0 mL·min^(-1),进样量10μL。结果莪术二酮、莪术醇、吉马酮、β-榄香烯4种挥发油线性关系良好(r≥0.9989),平均加样回收率≥93.67%。结论该方法简便快捷,适用于莪术油乳膏定量分析。Objective To establish a high performance liquid chromatography(HPLC)method for determination the main active components of zedoary turmeric oil cream.Methods The column was Agilent TC-C 18(4.6 mm×250 mm,5μm)at the temperature of 25℃.The mobile phase was composed of acetonitrile and water by gradient elution in the flow rate of 1.0 mL·min^(-1) with the detection wavelength of 216 nm.The injection volume was 10μL.Results The four compounds,curdione,curcumenol,germacrone,andβ-elemene,were well separated with good linear correlations(r≥0.9989).The average recoveries of four compounds were≥93.67%.Conclusion The HPLC method was simple,rapid and suitable for the quantitative analysis of zedoary turmeric oil cream.

关 键 词：莪术油乳膏 高效液相色谱法 含量测定 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]