基于DUS测试的玉米标准品种在贵州地区的表达差异及多样性分析  

Expression Differences and Diversity Analysis of Maize Standard Varieties Based on DUS Test in Guizhou

作　　者：李娟 孔德章 焦爱霞 周欢欢[2] 莫远琪 韦启迪 张丽娜 霍可以 LI Juan;KONG Dezhang;JIAO Aixia;ZHOU Huanhuan;MO Yuanqi;WEI Qidi;ZHANG Li'na;HUO Keyi(Guiyang Station for DUS Testing Center of New Plant Varieties,MARA,P.R.China,Guiyang 550006,China;Institute of Crop Germplasm Resources,Guizhou Academy of A gricultural Sciences,Guiyang 550006,China)

机构地区：[1]农业农村部植物新品种测试(贵阳)分中心,贵阳550006 [2]贵州省农业科学院农作物品种资源研究所,贵阳550006

出　　处：《种子》2021年第9期28-33,共6页Seed

基　　金：农业农村部物种品种资源保护费项目“物种品种资源保护费”[111721301354052309、111821301354052267];贵州省农业科学院青年基金“黔桂山区玉米品种DUS测试操作手册研制及标准品种鉴选”[黔农科院青年基金(2017)05号]。

摘　　要：植物品种DUS测试是品种授权、审定登记及认定、维权执法等的必要程序与重要手段。通过对玉米DUS测试指南中36份标准品种2018—2019年的田间测试,采用符合系数对标准品种在贵州地区的性状表现与玉米测试指南中的代码进行表达差异性分析,评价玉米标准品种在贵州地区的表达的准确性及形态多样性。结果表明,36个标准品种中有30个品种的符合系数大于0.95,占83.3%;39个测试性状中有30个性状的符合系数大于0.95,占76.9%,性状平均符合系数为质量性状(1)>假质量性状(0.982)>数量性状(0.967);39个测试性状在36份标准品种中检测到199个等位变异,变幅在1~9之间,平均为5.103个;有效等位变异数在1~6.694间,平均为3.282;Shannon-Weaver多样性指数介于0~2.008之间,平均为1.234;36份标准品种的形态相似系数范围为0.64~0.84,相似系数在0.81时,可将36份标准品种划分为31个类群,因此可将标准品种数量调减至31个。The DUS testing of plant variety is a necessary procedure and important means for variety authorization, registration and identification, and law enforcement of rights protection. Based on field tests conducted on 36 standard varieties in corn DUS test guidelines in 2018—2019, the difference between the traits of performance of standard varieties in Guizhou and the code in maize test guide was analyzed by coincidence coefficient, and the expression accuracy and morphological diversity of maize standard varieties in Guizhou were also evaluated. The results showed that 30 of the 36 standard varieties had coincidence coefficients greater than 0.95, accounting for 83.3%, while 30 of the 39 tested traits had coincidence coefficients greater than 0.95, accounting for 76.9%. And, the average coincidence coefficients were quality traits(1) > pseudoquality traits(0.982)> quantitative traits(0.967). The 199 allelic variations of 39 traits were detected in 36 standard varieties with a variable range of 1 to 9, with an average of 5.103. Additionally, the effective alleles ranged from 1 to 6.694, with an average of 3.282, and Shannon-weaver diversity index ranged from 0 to 2.008, with an average of 1.234. The morphological similarity coefficients of the 36 standard varieties ranged from 0.64 to 0.84, and the 36 standard varieties could be divided into 31 groups while the similarity coefficients were 0.81, so the number of standard varieties could be reduced to 31.

关 键 词：玉米 DUS测试 标准品种 符合系数 差异性分析 多样性分析 

分 类 号：S513[农业科学—作物学]