濒危植物曲茎石斛种子无菌萌发研究  

Study on Seed Germination of Endangered Plant Dendrobium flexicaule

作　　者：刘靖 胡泽方 程志杰 王仕沁 王玉兵 LIU Jing;HU Zefang;CHENG Zhijie;WANG Shiqin;WANG Yubing(Biotechnology Research Center of Three Gorges U niversity,Yichang Hubei 443002,China)

机构地区：[1]三峡大学生物技术研究中心,湖北宜昌443002

出　　处：《种子》2021年第9期48-51,56,F0002,共6页Seed

基　　金：神农架金丝猴保育生物学湖北省重点实验室开放基金(SNJGKL 202103)。

摘　　要：为保护与利用曲茎石斛野生资源,以自然授粉的曲茎石斛果实为研究材料,采用组织培养的方法,研究曲茎石斛种子的无菌萌发特性,探索基本培养基与外源添加物组合对种子萌发的影响,以筛选出最佳萌发培养基质。结果表明,曲茎石斛种子萌发经历吸胀阶段、原球茎形成阶段、叶形成阶段和生根阶段等4个阶段。曲茎石斛在以1/2 N_(6)为基本培养基,马铃薯泥为外源添加物时萌发率最高,可达98.8%,且萌发速度最快,14 d内即可开始萌发,84 d后成苗。本研究结果对于揭示曲茎石斛的保育和离体快速繁殖提供参考依据。In order to protect and utilize the wild resources of Dendrobium flexicaule, the seeds of naturally pollinated D. flexicaule were used as research materials, and the sterile germination characteristics of seeds of D. flexicaule were studied by tissue culture, and the effects of combination of basic medium and exogenous additives on seed germination were also explored, so as to screen out the optimal substrate for germination culture. The results showed that the seed germination of D. flexicaule underwent four stages, namely imbibition stage, procorm-formation stage, leaf-formation stage and rooting stage. The germination rate of D. flexicaule with 1/2 N_(6) as the basic medium and mashed potato as the exogenous additive reached 98.8%, and the germination rate was the fastest, and the germination could start within 14 days, and the seedlings formed after 84 days. The results of this study provide a reference for the conservation and rapid propagation of D. flexicaule in vitro.

关 键 词：曲茎石斛 种子 萌发特性 无菌萌发 

分 类 号：S567.239[农业科学—中草药栽培]