赤霉素浸种对柴胡种子发芽及内源激素变化的影响  

Effects of Soaking Seed with Gibberellin on Seed Germination and Endogenous Hormone Changes of Radix bupleuri

作　　者：袁梦佳 杨太新[1] 孙延超 YUAN Mengjia;YANG Taixin;SUN Yanchao(College of Agronomy,Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Crop Growth Regulation of Hebei Province,Baoding,Hebei 071001,China)

机构地区：[1]河北农业大学农学院/省部共建华北作物改良与调控国家重点实验室/河北省作物生长调控实验室,河北保定071001

出　　处：《种子》2021年第9期81-85,共5页Seed

基　　金：河北省中药材产业技术体系创新团队项目(HBCT 2018060202);太行山农业创新驿站安国药博园(1717050)。

摘　　要：为了研究不同浓度赤霉素浸种对柴胡种子发芽的影响,测定分析种子发芽过程中4种内源激素含量的变化差异,为柴胡种子萌发调控及提高种子发芽率提供理论依据和可行方法,采用0 mg·L^(-1)、50 mg·L^(-1)、100 mg·L^(-1)、150 mg·L^(-1)和200 mg·L^(-1)的赤霉素溶液浸种处理柴胡种子,高效液相色谱法测定种子发芽过程中ABA、GA3、IAA和ZR的含量。结果表明,不同浓度赤霉素浸种的柴胡种子发芽势和发芽率均差异显著,150 mg·L^(-1)浸种的发芽势(30.1%)和发芽率(64.5%)均最高。与对照相比,其他浸种处理种子发芽过程中的ABA、IAA含量均显著降低,GA3、ZR含量均显著增加。种子发芽过程中,各浸种的IAA+GA3+ZR/ABA比值均呈"上升-下降-上升"的变化,且其他浸种的该比值均显著高于对照组,以150 mg·L^(-1)浸种的最高。研究表明,柴胡种子发芽过程中ABA、GA3、IAA、ZR的含量呈现不同变化,赤霉素浸种可通过调节IAA+GA3+ZR/ABA比值上升,进而促进种子发芽。In order to study the effects of soaking seeds with different concentrations of gibberellin on seed germination of Radix bupleuri, to measure and analyze the variation of 4 endogenous hormone contents during seed germination process, and to provide theoretical basis and feasible method for seed germination regulation and seed germination rate improvement of R. bupleuri, the seeds were treated with 0 mg·L^(-1), 50 mg·L^(-1), 100 mg·L^(-1), 150 mg·L^(-1) and 200 mg·L^(-1) gibberellin solution, and the contents of ABA, GA3, IAA and ZR in seeds during germination were determined by HPLC. The results showed that the germination potential and germination rate of R. bupleuri seeds soaked with different concentrations of gibberellin were significantly different, and the germination potential(30.1%) and germination rate(64.5%) of seeds soaked with 150 mg·L^(-1) were the highest. Compared with the control, the contents of ABA and IAA in other soaking treatments were significantly decreased, while the contents of GA3 and ZR were significantly increased. During the germination process, IAA+GA3+ZR/ABA ratio of all soaking seeds showed a "rise-decline-rise" change, and the ratio of other soaking seeds was significantly higher than that of control group, and the highest value was 150 mg·L^(-1) soaking seeds. The results showed that the contents of ABA, GA3, IAA and ZR in seeds of R. bupleuri showed different changes during the germination process, and gibberellin soaking seeds may promote seed germination by regulating the increase of IAA+GA3+ZR/ABA ratio.

关 键 词：柴胡 赤霉素 种子发芽 内源激素 

分 类 号：S567.79[农业科学—中草药栽培]