丹参种子活力快速测定方法的建立及其与萌发率的相关性  

Establishment of A Rapid Determination Method for Seed Vigor of Salvia Miltiorrhiza Bunge and Its Correlation with Germination Rate

作　　者：廖芳 任瑞花 孔德英 刘伟[2] 冯洁 韩艺 李关荣[2] LIAO Fang;REN Ruihua;KONG Deying;LIU Wei;FENG Jie;HAN Yi;LI Guanrong(Animal,Plant and Foodstuff Inspection Center,Tianjin Customs,Tianjin 300461,China;College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,China;Technology Center,Chongqing Customs,Chongqing 401333,China)

机构地区：[1]天津海关动植物与食品检测中心,天津300461 [2]西南大学农学与生物科技学院,重庆400716 [3]重庆海关技术中心,重庆401333

出　　处：《种子》2021年第9期130-133,共4页Seed

基　　金：国家重点研发计划(2017 YFF 0210303、2017 YFF 0210305)。

摘　　要：本研究采用溴麝香草酚蓝(BTB)法快速测定丹参种子活力,通过类原位萌发与常规萌发试验,研究BTB快速测定种子活力与萌发率之间的相关性。结果表明,丹参种子类原位萌发率(y1)与BTB法测得的种子活力(x)的回归方程为y1=0.983 5 x1+1.859 5,R_(1)^(2)=0.996,常规萌发率(y2)与BTB法测得的种子活力(x)的回归方程为y2=0.347 1 x2+51.95,R_(2)^(2)=0.972,表明BTB法可用于快速测定丹参种子活力并预估其萌发率。The seeds of Chinese traditionul medicine are very small, their maturity is in great disparity. the germination rate is usually low under natural conditions, and the initial growth of the seedlings is very slow. which makes it dffcult to estimate the seed amount and to ensure sufficient and robust plants needed for cultivation in large scales. In this study. the bromothymol blue(BTB) method was used to rnpid determine the seed vigor of the W-SCHY-W-1 population of, and the relationships between seed vigor and the germination rates from the in situ-like and conventional germination tests were established. Results showed that the regression equation of in situ-like grmination rate(y) with seed vigor determined by BTB(x) is y=0.983 5 x+1.859 5. R^(2)=0.996, and the regression equation of conventional germination rate(y) and seed vigor(x) is y= 0,347 1 x+51.95, R^(2)=0.972. In conclusion, BTB method is applicable for rapid determination of the seed vigor and prediction of the gertmination rates of seeds.

关 键 词：丹参 种子活力快速测定 BTB法 类原位萌发 常规萌发 相关性 

分 类 号：S567.53[农业科学—中草药栽培]