利用COMPASS和MYP培养基检测雪茄烟叶中蜡样芽胞杆菌  

Detection of Bacillus cereus in cigar tobacco leaves using COMPASS and MYP media

作　　者：叶长文[1] 罗凯玉 陈宸[1] 贾楠[1] 李栋[1] 李青常[1] 范黎[1] 贺琛[1] YE Changwen;LUO Kaiyu;CHEN Chen;JIA Nan;LI Dong;LI Qingchang;FAN Li;HE Chen(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;Tobacco College,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州市450001 [2]河南农业大学烟草学院,郑州市450002

出　　处：《烟草科技》2021年第10期11-19,共9页Tobacco Science & Technology

基　　金：中国烟草总公司郑州烟草研究院院长科技发展基金项目“手工雪茄烟微生物污染状况分析研究”(No.702017CA0190)。

摘　　要：为探索一种适宜雪茄烟叶中蜡样芽胞杆菌(Bacillus cereus)检测的快速准确方法,比较了COMPASS显色培养基法(COMPASS方法)和ISO 7932两种方法的性能。结果表明:(1)采用标准菌株和定量质控菌株分别验证COMPASS显色培养基和ISO 7932方法用甘露醇卵黄多黏菌素琼脂(MYP)培养基的性能,COMPASS显色培养基的特异性和选择性更好,灵敏度与ISO 7932方法相当。(2)两种方法对人工污染样品和自然样品中蜡样芽胞杆菌检测的结果差异不显著(P>0.05),而COMPASS方法操作简单,检测周期短、准确性更高。(3)24份雪茄烟叶样品中蜡样芽胞杆菌的检出率为83.3%,检出量皆小于1×10^(5)CFU/g。因此,COMPASS方法优于ISO 7932方法,可有效减少实际样品中非目标芽胞杆菌干扰,适用于雪茄烟叶中蜡样芽胞杆菌的快速检测。In order to find a fast and accurate method for the determination of Bacillus cereus in cigar tobacco leaves,the performances of COMPASS chromogenic medium method(COMPASS method)and the ISO 7932 method were compared.The results showed that:1)The standard strain and quantitative quality control strain were used to verify the performances of COMPASS chromogenic medium and mannitol yolk polymyxin agar(MYP)medium used in the ISO 7932 method.The COMPASS chromogenic medium was more specific and selective than MYP medium,while their sensitivities were similar.2)The difference between the detection results of the two methods on artificially contaminated samples and natural samples was not significant(P>0.05),but the COMPASS method was simpler in use,needed less time and offered a higher accuracy.3)The detection rate of Bacillus cereus in 24 cigar tobacco leaf samples was 83.3%with the level less than 10^(5) CFU/g.Therefore,the COMPASS method was considered superior to the ISO 7932 method,as it could effectively reduce the interference of non-target Bacillus spp.in actual samples.In conclusion,the COMPASS method is suitable for the rapid detection of Bacillus cereus in cigar tobacco leaves.

关 键 词：雪茄烟叶 蜡样芽胞杆菌 COMPASS MYP 显色培养基 

分 类 号：TS414[农业科学—烟草工业]