过山蕨抗炎化学成分的分离鉴定  

Isolation and Identification of Anti⁃inflammatory Chemical Constituents of Asplenium ruprechtii Herb

作　　者：王芳 张宁 张岱州 李敏 袁振海 段崇刚 梁大连 WANG Fang;ZHANG Ning;ZHANG Dai-zhou;LI Min;YUAN Zhen-hai;DUAN Chong-gang;LIANG Da-lian(Shandong Academy of Pharmaceutical Sciences/Shandong Key Laboratory of Chemical Medicines,Jinan 250101,China)

机构地区：[1]山东省药学科学院/山东省化学药物重点实验室,山东济南250101

出　　处：《中药材》2020年第12期2928-2932,共5页Journal of Chinese Medicinal Materials

基　　金：山东省重点研发计划(2016GSF202006);国家重大新药创制(2017ZX09301003)。

摘　　要：目的:研究过山蕨Asplenium ruprechtii Sa.Kurata的化学成分,并测定其抗炎活性。方法:通过正相硅胶、MCI树脂、Sephadex LH⁃20凝胶、制备薄层色谱和反相HPLC等多种色谱技术进行分离纯化,通过波谱数据分析,结合文献鉴定化合物的结构。结果:从过山蕨中共分离得到12个化合物,分别鉴定为:山柰酚⁃3⁃O⁃[(6⁃咖啡酰基)⁃β⁃D⁃吡喃葡萄糖基]⁃(1→2)⁃β⁃D⁃吡喃葡萄糖苷(1)、山柰酚⁃3⁃O⁃[(6⁃咖啡酰基)⁃β⁃D⁃吡喃葡萄糖基]⁃(1→2)⁃β⁃D⁃吡喃葡萄糖基⁃7⁃O⁃β⁃D⁃吡喃葡萄糖苷(2)、山柰酚⁃3⁃O⁃[(6⁃香豆酰基)⁃β⁃D⁃吡喃葡萄糖基]⁃(1→2)⁃β⁃D⁃吡喃葡萄糖基⁃7⁃O⁃β⁃D⁃吡喃葡萄糖苷(3)、elaphoside A(4)、ptetatoside A(5)、山柰酚⁃3⁃O⁃[(6⁃阿魏酰基)⁃β⁃D⁃吡喃葡萄糖基]⁃(1→2)⁃β⁃D⁃吡喃半乳糖苷(6)、槲皮素⁃3⁃O⁃[(6⁃阿魏酰基)⁃β⁃D⁃吡喃葡萄糖基]⁃(1→2)⁃β⁃D⁃吡喃葡萄糖苷(7)、(E)⁃6⁃O⁃香豆酰鸡屎藤次苷甲酯(8)、(Z)⁃6⁃O⁃香豆酰鸡屎藤次苷甲酯(9)、七叶苷(10)、5′⁃O⁃甲基腺嘌呤核苷(11)、鸟嘌呤核苷(12)。结论:其中,化合物6~12为首次从铁角蕨属植物中分离得到。对分离的12个化合物采用Griess法检测脂多糖(LPS)诱导的RAW264.7巨噬细胞培养液中NO的含量进行了抗炎活性研究,结果显示化合物1~3、6~10对LPS诱导的RAW264.7巨噬细胞NO的释放均显示有一定抑制活性;在1.0×10^(-4)mol/L浓度下,其他化合物均未表现出活性。Objective:To study the chemical constituents of Asplenium ruprechtii and determine its anti⁃inflammatory activities.Methods:The compounds were isolated and purified by a combination of various chromatographic techniques,including posi⁃tive phase silica gel,MCI,Sephadex LH⁃20,thin layer chromatography and reverse phase HPLC.The structures were identified on the basis of spectral data analysis and literature.Results:Twelve compounds were isolated from Asplenium ruprechtii,and identified as kaempferol⁃3⁃O⁃[(6⁃O⁃E⁃caffeoyl)⁃β⁃D⁃glucopyranosyl]⁃(1→2)⁃β⁃D⁃glucopyranoside(1),kaempferol⁃3⁃O⁃[(6⁃O⁃E⁃caffeoyl)⁃β⁃D⁃glucopyranosyl]⁃(1→2)⁃β⁃D⁃glucopyranosyl⁃7⁃O⁃β⁃D⁃glucopyranoside(2),kaempferol⁃3⁃O⁃[(6⁃O⁃p⁃coumaroyl)⁃β⁃D⁃glucopyrano⁃syl]⁃(1→2)⁃β⁃D⁃glucopyranosyl⁃7⁃O⁃β⁃D⁃glucopyranoside(3),elaphoside A(4),ptetatoside A(5),kaempferol⁃3⁃O⁃[(6⁃O⁃E⁃feru⁃loyl)⁃β⁃D⁃glucopyranosyl]⁃(1→2)⁃β⁃D⁃galacopyranoside(6),quercetin⁃3⁃O⁃[(6⁃O⁃E⁃feruloyl)⁃β⁃D⁃glucopyranosyl]⁃(1→2)⁃β⁃D⁃glucopyranoside(7),(E)⁃6⁃O⁃p⁃coumaroyl scandoside methyl ester(8),(Z)⁃6⁃O⁃p⁃coumaroyl scandoside methyl ester(9),aesculin(10),5′⁃O⁃methyladenosine(11),guanosine(12),respevtively.Conclusion:Among them,compounds 6 to 12 are isolated from Aspleni⁃um genus for the first time.The anti⁃inflammatory activities of NO in RAW264.7 macrophage culture induced by lipopolysaccharide(LPS)are determined by Griess assay for 12 compounds.The results show that compounds 1 to 3,6 to 10 show certain inhibitory activi⁃ties on release of NO in RAW264.7 macrophages induced by LPS.At 1.0×10^(-4)mol/L,none of the other compounds show activities.

关 键 词：过山蕨 化学成分 结构鉴定 抗炎活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]