QuEChERS/超高效液相色谱-串联质谱法测定黑木耳中33种除草剂残留  

Simultaneous Determination of 33 Herbicides Residues in Auricularia auricula by QuEChERS and Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：李杨 平华[1] 李冰茹 孔红玲 李成 马智宏[1] LI Yang;PING Hua;LI Bing-ru;KONG Hong-ling;LI Cheng;MA Zhi-hong(Beijing Research Center for Agricultural Standards and Testing,Beijing Academy of Agriculture and Forestry Sciences,Risk Assessment Lab for Agro-products(Beijing),Ministry of Agriculture P.R.China,Beijing 100097,China)

机构地区：[1]北京市农林科学院北京农业质量标准与检测技术研究中心农业部农产品质量安全风险评估实验室(北京),北京100097

出　　处：《农药》2021年第10期751-755,共5页Agrochemicals

基　　金：北京农业质量标准与检测技术研究中心课题(ZBZXCX201901);北京市农林科学院青年基金(QNJJ202116)。

摘　　要：[目的]建立一种基于QuEChERS(Quick Easy Cheap Effective Rugged and Safe)前处理技术和超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定黑木耳中33种除草剂残留量的分析方法。[方法]黑木耳提取液采用分散固相萃取净化,UPLC-MS/MS测定,电喷雾电离,多反应监测模式检测。[结果]33种除草剂在1.0(2.0)~100(200)μg/L质量浓度范围内线性关系良好,相关系数均不小于0.9990;3个加标水平(0.020、0.040、0.200 mg/kg)的平均回收率为72.2%~122.7%,相对标准偏差(RSD)为0.8%~13.4%;检出限为0.003~0.015 mg/kg。[结论]该方法简单、快速、准确、灵敏度高,可用于大批黑木耳中除草剂残留快速检测。[Aims] This study aims to establish a method for simultaneous determination of 33 herbicide residues in Auricularia auricular by QuEChERS(Quick Easy Cheap Effective Rugged and Safe) and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). [Methods] The extract of A. auricular sample was purified by QuEChERS method. UPLC-MS/MS method was performed with electrospray ionization(ESI) source under the multiple reaction monitoring(MRM) mode. [Results] The linear correlation coefficients were no less than the relative Standard devia tion(RSD) ranged from 0.8 to 13.4% at 0.9990 with the concentration range of 1.0(2.0)-100(200) μg/L.The average recoveries ranged from 72.2 to 122.7% and the relative standard deviation(RS) ranged from 0.8 to 13.4% at the addition levels of 0.020, 0.040 and 0.200 mg/kg. The limits of detection ranged from 0.003 to 0.015 mg/kg.[Conclusions] This method is easy operation, rapid, good sensitivity and accuracy, which is suitable for the simultaneous detection of herbicide residues in a large number of A. auricular samples.

关 键 词：黑木耳 除草剂 QUECHERS 超高效液相色谱-串联质谱(UPLC-MS/MS) 

分 类 号：TQ450.2[化学工程—农药化工]