橡胶树前纤维蛋白(profilin)基因家族的鉴定及表达分析  

Identification and Expression Analysis of profilin Gene Family in Hevea brasiliensis

作　　者：邓治[1] 李德军[1] DENG Zhi;LI Dejun(Rubber Research Institute,Chinese Academy of Tropical Agriculture Sciences/Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs/State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南海口571101

出　　处：《热带作物学报》2021年第9期2443-2450,共8页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院基本科研业务费专项(No.1630022019018);国家重点研发计划项目(No.2018YFD1000502);海南省自然科学基金项目(No.718MS038)。

摘　　要：肌动蛋白细胞骨架可能在橡胶树乳管伤口堵塞过程中发挥重要作用。前纤维蛋白(profilin)是肌动蛋白动态平衡的重要调节子,但对橡胶树profilin基因家族系统研究的报道较少。通过分析橡胶树基因组和转录组数据,鉴定到6个橡胶树profilin基因,对其基本特性及蛋白保守基序、结构特征、进化关系和表达模式等进行分析。基因结构分析表明,橡胶树profilin基因都包含3个外显子2个内含子,编码的蛋白序列含有profilin蛋白特有的保守基序KYMVIQGE和VIRGKKG。进化分析显示,橡胶树profilin并未严格分为营养型和生殖型2种类型。profilin蛋白二级结构以无规则卷曲为主,三级结构均包含3个α螺旋和7个β折叠。表达分析结果显示,4个profilin基因在胶乳中高表达,橡胶树排胶和碘化钾处理调控这4个profilin基因表达,推测profilin基因参与橡胶树排胶过程。该研究结果为进一步阐明橡胶树profilin基因在乳管伤口堵塞和排胶中的作用奠定基础。Actin cytoskeleton might play an important role in laticifer wound plugging of Hevea brasiliensis.Although profilin is a vital regulator in actin dynamics,there is still no systematic study on profilin gene family in Hevea brasiliensis.In this study,six profilin genes were identified from the genome and transcriptome of Hevea brasiliensis.The six genes were analyzed in details,including gene basic characteristics,conserved protein motifs,structure features,evolutionary relationships,and expression profiles.Gene structure analyses demonstrated that the six profilin genes contained three exons and two introns.Conserved domain analyses indicated that the six profilin proteins possessed unique motifs of profilin protein,KYMVIQGE and VIRGKKG motifs.Evolutionary analyses showed that Hevea profilins were not strictly divided into vegetative and reproductive subclasses.The main secondary structures of profilin proteins were random coil and tertiary structures consist of threeαhelices and sevenβturns.Expression analyses showed that four profilin genes were highly expressed in latex,and regulated by latex flow and potassium iodide treatment,suggesting that profilin genes might be involved in latex flow.These results would lay a foundation for further elucidating profilin roles in laticifer wound plugging and latex flow of Hevea brasiliensis.

关 键 词：前纤维蛋白基因家族 橡胶树 排胶 表达分析 

分 类 号：S794.1[农业科学—林木遗传育种]