拟南芥mapkkk15突变体的鉴定及非生物胁迫下的功能分析  

Identification of mapkkk15 Mutant from Arabidopsis and Function Analysis to Abiotic Stress

作　　者：梁群 邓治[2] 雷柯煜 黄华孙[2] 安泽伟[2] 程汉[2] LIANG Qun;DENG Zhi;LEI Keyi;HUANG Huasun;AN Zewei;CHENG Han(College of Tropical Crops,Hainan Unviersity,Haikou,Hainan 570228,China;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)

机构地区：[1]海南大学热带作物学院,海南海口570228 [2]中国热带农业科学院橡胶研究所,海南海口571101

出　　处：《热带作物学报》2021年第9期2494-2500,共7页Chinese Journal of Tropical Crops

基　　金：国家重点研发计划项目“橡胶树抗寒高产品种改良”(No.2019YFD1001102);国家自然科学基金项目(No.31870646)。

摘　　要：低温寒害是制约我国天然橡胶种植的最主要的环境限制因子,阐明橡胶树抗逆机制有助于保障天然橡胶的种植安全。前期研究发现1个低温诱导的橡胶树MAPKKK基因参与橡胶树抗寒能力调控,序列比对发现该基因与拟南芥MAPKKK15基因同源,但AtMAPKKK15的功能仍不清楚。通过对拟南芥MAPKKK15基因功能的研究,揭示该类基因在植物逆境胁迫应答中的作用,将有助于进一步解析橡胶树MAPKKK基因的功能。本研究从DNA和转录水平鉴定拟南芥mapkkk15纯合突变体植株,评价mapkkk15突变体低温和干旱胁迫抗性。结果显示:低温抑制AtMAPKKK15基因表达。对2个mapkkk15纯合缺失突变体进行分析,发现与野生型植株相比,mapkkk15突变体植株的抗冻存活率提高,电解质渗漏率下降。脱水实验表明,突变体叶片脱水率要高于野生型。上述结果表明,AtMAPKKK15基因在拟南芥中可能反向调控抗寒性,正向调控抗旱性。Abiotic stress severely affects the natural rubber yield in China,so it is necessary to clarify the resistant mechanism of rubber tree to improve natural rubber yield.In previous studies,we found that a MAPPKKK was induced by low temperature in rubber tree.Sequence alignment showed that HbMAPKKK was homologous with MAPKKK15 from Arabidopsis.However,the function of AtMAPKKK15 is unclear.Function of AtMAPKKK15 was identified in responding to stress and provide theoretical basis for further elucidating the function of MAPKKK in rubber tree.The homozygous T-DNA insertion mutants of AtMAPKKK15 were identified at DNA and transcription level,and evaluated resistance under low temperature and drought treatments.The results showed that the expression of AtMAPKKK15 was inhibited by low temperature.Two homozygous null mutants of MAPKKK15 were obtained from Arabidopsis.The mapkkk15 mutant improved low temperature tolerance by increasing survival and decreasing electrolyte leakage compared with the wild-type.However,the dehydration ration of mutant leaves increased with the extension of in vitro time.The results indicate that MAPKKK15 negatively regulated tolerance to low temperature and postively regulated resisitance to drought.

关 键 词：mapkkk15突变体 拟南芥 非生物胁迫 功能鉴定 橡胶树 

分 类 号：S961.6[农业科学—水产养殖]