水心病菠萝果实生理指标和内源激素含量变化  

Changes of Physiological Indexes and Endogenous Hormone Content of Watercore Pineapple Fruit

作　　者：姚艳丽[1,2,3,4] 付琼 周迪 朱祝英 杨玉梅 张秀梅 YAO Yanli;FU Qiong;ZHOU Di;ZHU Zhuying;YANG Yumei;ZHANG Xiumei(South Subtropical Crop Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,Guangdong 524091,China;Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture and Rural Affairs,Zhanjiang,Guangdong 524091,China;Hainan Provincial Engineering Research Center for Pineapple Germplasm Innovation and Utilization,Zhanjiang,Guangdong 524091,China;ZhanJiang Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,Guangdong 524091,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究,广东湛江524091 [2]农业农村部热带果树生物学重点实验室,广东湛江524091 [3]海南省菠萝种质创新与利用工程技术研究中心,广东湛江524091 [4]中国热带农业科学院湛江实验站,广东湛江524091

出　　处：《热带作物学报》2021年第9期2587-2593,共7页Chinese Journal of Tropical Crops

基　　金：国家重点研发计划项目(No.2019YFD1000505);所基本科研业务费项目(No.1630102020002);广东省自然科学基金项目(No.2019A1515011186)。

摘　　要：为了解水心病菠萝果实生理指标和内源激素含量的变化特征,本研究以菠萝主栽品种‘巴厘’为材料,采用液质联用(LC-MS)、高效液相色谱(HPLC)等方法测定了不同发病程度的水心病菠萝果实中主要内源激素、单糖、可溶性固形物、可滴定酸和维生素C(Vc)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,并分析了水心病发病程度、内源激素和抗氧化酶活性间的相关性。结果表明,不同发病程度水心病菠萝可溶性固形物、Vc和糖含量、SOD和POD酶活性以及内源激素玉米素(ZT)、茉莉酸(JA)和脱落酸(ABA)含量均比对照显著下降,且随着发病程度的加深下降幅度增加;不同发病程度水心病菠萝CAT酶活性、MDA含量和SA含量较对照显著提高;可滴定酸含量、果糖和葡萄糖含量在轻度发病果中含量较对照明显增加,在中度发病果中含量显著降低,而1-氨基环丙烷羧酸(ACC)含量变化趋势与其刚好相反。相关分析表明,菠萝水心病病害级别与内源激素ZT、JA和ABA含量呈显著负相关,相关系数分别为–0.98、–0.98和–0.94;与SA含量呈显著正相关,相关系数为0.93。一方面水心病降低了菠萝果实糖酸比和Vc含量,导致果实品质下降;另一方面菠萝通过调节内源激素ZT、JA、ABA和SA含量,进而调控SOD、POD和CAT活性,增强对水心病的防御反应。In order to understand the variation characteristics of physiological indexes and endogenous hormone content of watercore pineapple fruit,the main pineapple cultivar“Bali”was used as the material in this study,and the content of the endogenous hormones,monosaccharides,soluble solids,titratable acids,vitamin C(Vc),malondialdehyde(MDA),the activity of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)was determined by liquid chromatography-mass spectrometry(LC-MS),high-performance liquid chromatography(HPLC)and so on.The correlation among the degree of watercore,endogenous hormone and antioxidant enzyme activity was analyzed.The results showed that the content of soluble solids,Vc and sugar,the activity of SOD and POD and the content of ZT,JA and ABA decreased significantly in different degrees of watercore,and the decreasing extent increased with the deepening of disease degree.The activity of CAT,the content of MDA and SA in different degrees of watercore were significantly higher than those in the control.Titratable acid content,fructose and glucose content in mild watercore fruit increased significantly compared with the control,and decreased significantly in moderate watercore fruit,while the variation trend of 1-aminocyclopropane carboxylic acid(ACC)content was just opposite to them.The correlation analysis showed that the degree of watercore was negatively correlated with ZT,JA and ABA contents,the correlation coefficients were-0.98,-0.98 and-0.94,respectively,and positively correlated with SA contents,the correlation Coefficient was 0.93.

关 键 词：菠萝 水心病 品质 内源激素 抗氧化酶 

分 类 号：S210.4[农业科学—农业机械化工程]