松嫩盐碱草地紫花地丁根围AM真菌多样性  

Investigation of AM Fungal Diversity in the Rhizosphere of Viola philippica in Songnen Saline-Alkali Grassland

作　　者：赵文娜 杨春雪[1] Zhao Wenna;Yang Chunxue(Northeast Forestry University,Harbin 150040,P.R.China)

机构地区：[1]东北林业大学园林学院,哈尔滨150040

出　　处：《东北林业大学学报》2021年第9期83-89,共7页Journal of Northeast Forestry University

基　　金：国家自然科学基金青年科学基金项目(31601986);中央高校基本科研业务费专项资金项目(2572018BK02);黑龙江省博士后科研启动金项目(LBH-Q16005)。

摘　　要：采用形态学鉴定和高通量测序技术结合的方法,对松嫩盐碱草地紫花地丁根围土壤中AM真菌进行调查。形态学共鉴定出11属30种AM真菌,其中球囊霉属(Glomus)种类最多,共9种。以科学性为前提的基础上,为进一步深入研究紫花地丁根围AM真菌种类,通过高通量测序共得到7属103种虚拟种(Virtual taxa,VT):球囊霉属共82种,近明球囊霉属(Claroideoglomus)共10种,类球囊霉属(Paraglomus)共5种,多样孢囊霉属(Diversispora)共3种,盾巨孢囊霉属(Scutellospora)、无梗囊霉属(Acaulospora)、双型囊霉属(Ambispora)各1种。进一步得到了球囊霉属为松嫩盐碱草地紫花地丁根围土壤AM真菌优势属,Glomus sp.VTX00222为优势种。通过形态学鉴定和高通量测序技术相结合,弥补单种方法的局限性,提高结果的科学性和多样性。By combining morphological identification and high-throughput sequencing,we investigated the AM fungi in the rhizosphere of Viola philippica in Songnen saline-alkali grassland.A total of 30 AM fungal species of 11 genera were identified by morphologically,including 9 species of Glomus.By exploring AM fungal diversity in the rhizosphere of V.philippica,103 virtualtaxa(VT)of 7 genera were obtained by high-throughput sequencing,including 82 VT of Glomus,10 VT of Claroideoglomus,5 VT of Paraglomus,3 VT of Diversispora,and 1 VT of Scutellospora,Acaulospora,Ambispora,respectively.Among which Glomus was the dominant genus of AM fungi in the rhizosphere of V.philippica in Songnen saline-alkali grassland,and the Glomus sp.VTX00222 was the dominant species.Through the combination of morphological identification and high-throughput sequencing technology,the limitation of single method was made up,and the scientificity and diversity of results were improved.

关 键 词：蓝紫色花 AM真菌多样性 高通量测序技术 形态学鉴定 盐碱地 

分 类 号：S682[农业科学—观赏园艺] Q939.5[农业科学—园艺学]