响应面法优化超临界CO2萃取红参多糖工艺及体外抗氧化活性研究  

Optimization of supercritical CO2 extraction of red ginseng polysaccharide by response surface methodology and its antioxidant activity in vitro

作　　者：孙婧[1] 孟欣欣 景亚 宋艺君[1] 杨巧 Sun Jing;Meng Xinxin;Jing Ya;Song Yijun;Yang Qiao(College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China;College of MedicalTechnology,the Medicine College of Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学药学院,咸阳712046 [2]陕西中医药大学医技学院,咸阳712046

出　　处：《国际中医中药杂志》2021年第9期887-892,共6页International Journal of Traditional Chinese Medicine

基　　金：陕西中医药管理局中药炮制学重点学科资助项目(132018003);陕西省体育局项目(2020261)。

摘　　要：目的采用响应面法优化红参多糖的最佳提取工艺条件,研究红参多糖的抗氧化活性。方法采用超临界CO2萃取法提取红参多糖,考察料液比、萃取时间、萃取温度、萃取压力对红参多糖提取的影响。运用Box-Behnken Design法对红参多糖提取工艺进行优化,采用Logit法计算红参多糖对DPPH清除率的半抑制浓度(half maximal inhibitory concentration,IC50),研究其抗氧化活性。结果以红参多糖得率为评价指标,最优提取条件为萃取温度61.12℃、萃取压力20.64 MPa、萃取时间128.37 min、料液比1∶25.61 g/ml,红参多糖得率为36.89%。3组重复性试验结果显示,该条件下红参多糖得率相对误差在5%范围内。红参多糖质量浓度为25μg/ml时有较好的抗氧化活性,IC50为10.97μg/ml。结论优化的红参多糖提取工艺条件合理可靠,红参多糖抗氧化活性较强,可为后续研究提供参考。Objective Response surface methodology was used to optimize the optimal extraction conditions of red ginseng polysaccharide and to study the antioxidant activity of red ginseng polysaccharide.Methods Supercritical CO2 extraction method was used to extract polysaccharides from red ginseng.The effects of solid-liquid ratio,extraction time,extraction temperature and extraction pressure on the extraction of polysaccharides from red ginseng were investigated.Box-behnken Design method was used to optimize the extraction process of red ginseng polysaccharide,and Logit method was used to calculate the semi-inhibitory concentration of red ginseng polysaccharide on DPPH clearance(half maximal inhibitory concentration,IC50).Results The optimal extraction conditions were as follows:extraction temperature 61.12℃,extraction pressure 20.64 MPa,extraction time 128.37 min,solid-liquid ratio 1∶25.61 g/ml,and the extraction yield of red ginseng polysaccharide was 36.89%.The results of three groups of repeatability tests showed that the relative error of polysaccharide yield of red ginseng was in the range of 5%.When the mass concentration of red ginseng polysaccharide was 25μg/ml,it had better antioxidant activity and IC50 was 10.97μg/ml.Conclusion The optimized extraction conditions of red ginseng polysaccharide were reasonable and reliable,and the antioxidant activity of red ginseng polysaccharide was strong,which could provide reference for the follow-up research.

关 键 词：红参多糖 超临界CO2 萃取 响应面法 

分 类 号：R284.2[医药卫生—中药学]