针刺对脑出血大鼠血红素氧化酶1及炎性因子表达的影响  

Effect of Acupuncture on the Expression of Heme Oxygenase 1 and Inflammatory Factors in Rats with Intracerebral Hemorrhage

作　　者：陈秋欣[1,2] 孔莹[3] 于婷婷[1] 张瑜 刘鹏[1] 张鑫[1] CHEN Qiuxin;KONG Ying;YU Tingting;ZHANG Yu;LIU Peng;ZHANG Xin(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Haerbin,Heilongjiang 150040,China;Heilongjiang University of Chinese Medicine,Haerbin,Heilongjiang 150040,China;The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Haerbin,Heilongjiang 150001,China;Shenzhen Baoan Hospital of Traditional Chinese Medicine,Shenzhen,Guangdong 518133,China)

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150001 [3]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [4]深圳市宝安中医院(集团),广东深圳518133

出　　处：《康复学报》2021年第5期408-414,共7页Rehabilitation Medicine

基　　金：国家自然科学基金项目(82004442);黑龙江省自然科学基金项目(H2018062)。

摘　　要：目的:从行为学、组织学及分子生物学层次分别探讨针刺百会透曲鬓穴对急性脑出血大鼠血红素氧化酶1(HO-1)及炎性因子表达的影响,为临床针刺治疗脑出血提供实验基础。方法:将108只Wistar雄性大鼠按照随机数字表法分为假手术组、模型组和针刺组,每组按1、3、7 d时间点再分为3个亚组,每个亚组各12只。自体血注入法建立脑出血大鼠模型,通过Berderson评分法确定动物成功模型,评分1~3分的大鼠纳入实验。假手术组接受类似模型组的各项手术操作,但不进行注血制作;模型组接受脑出血模型制作,但不进行任何干预;针刺组制作脑出血模型,于造模后12 h开始治疗。采用针刺百会透曲鬓穴对脑出血大鼠治疗,进针深度20 mm,以100 r/min小幅度捻转。每间隔5 min捻针1次,每次持续捻转5 min,留针30 min,每日1次。采用改良的神经功能缺损评分(mNSS)对各组大鼠神经功能进行评估,通过HE染色观察各组大鼠脑组织病理形态学变化,并采用Western blot法检测各组大鼠血肿组织HO-1、核转录因子-κB(NF-κB)、白介素1β(IL-1β)、转化生长因子-β(TGF-β)蛋白表达。结果:(1)假手术组各时间点无明显神经功能缺损表现;模型组大鼠于第1天时已表现出神经功能缺损症状,第3天及第7天时仍有明显神经缺损表现;与模型组相比,针刺组在各时间点大鼠神经功能评分均明显降低(P<0.01)。(2)假手术组大鼠基底节区可见正常的神经细胞及胶质细胞;模型组大鼠在造模第1天后可见出血灶,第3天出血灶增大,脑组织病理结构破坏明显,第7天血肿范围减少;与模型组相比,针刺组在各时间点脑组织病理结构破坏程度降低。(3)假手术组可见HO-1蛋白少量表达;模型组各时间点HO-1蛋白表达增多;与模型组相比,针刺组于第3天、第7天HO-1蛋白表达明显升高(P<0.05)。(4)假手术组均可见NF-κB、IL-1β、TGF-β蛋白少量表达;模型组各时间点Objective:To explore the effects of acupuncture at Baihui through Qubin acupoint on the expression of heme oxygenase 1(HO-1)and inflammatory factors in rats with acute intracerebral hemorrhage from the levels of behavior,histology and molecular biology,so as to provide an experimental basis for clinical acupuncture in the treatment of intracerebral hemorrhage.Methods:A total of 108 Wistar male rats were randomly divided into sham operation group,model group,acupuncture+model group(referred to as acupuncture group).Each group was divided into 3 subgroups according to 1 d,3 d and 7 d,with 12 rats in each group.The rat model of intracerebral hemorrhage was established by autologous blood injection.The sham operation group received various surgical operations similar to the model group,but no blood injection was performed;the model group received intracerebral hemorrhage model production without any intervention;the acupuncture group was used to create a intracerebral hemorrhage model,and treatment began 12 hours after the model was created.The Berderson scoring method was used to determine the animal success model,and rats with a score of 1-3 were included in the experiment.The rats with intracerebral hemorrhage in the acupuncture group were treated by acupuncture Baihui through Qubin acupoint.The depth of the needle was 20 mm,and the needle was twirled at 100 r/min with small amplitude.Twist the needle once every 5 minutes,and keep the needle for 30 minutes each time,once a day.Modified Neurological Severity Score(mNSS)was used to evaluate the neurological function of rats after intracerebral hemorrhage on 1 d,3 d and 7 d.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of brain tissue.The expression of HO-1,nuclear transcription factor-κB(NF-κB),Interleukin-1β(IL-1β),transforming growth factor-β(TGF-β)protein in hematoma tissue was detected by Western blot method at three time points of 1 d,3 d and 7 d.Results:1)There were no obvious neurological deficits in the sham operation group

关 键 词：脑出血 针刺 神经功能 血红素氧化酶1 炎性因子 

分 类 号：R245[医药卫生—针灸推拿学]