电针百会、神庭对系统炎症诱导认知功能障碍的海马抗炎作用  

Hippocampal Anti-inflammatory Effect of Electroacupuncture at Baihui and Shenting on Cognitive Dysfunction Induced by Systemic Inflammation

作　　者：陈小梅 庞莉娜 王志福[2] CHEN Xiaomei;PANG Lina;WANG Zhifu(College of Rehabilitation Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;College of Integrated Chinese and Western Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China)

机构地区：[1]福建中医药大学康复医学院,福建福州350122 [2]福建中医药大学中西医结合学院,福建福州350122

出　　处：《康复学报》2021年第5期415-419,424,共6页Rehabilitation Medicine

基　　金：国家自然科学基金面上项目(81774385);国家自然科学基金青年科学基金项目(81704149);福建省自然科学基金项目(2018J01879)。

摘　　要：目的:探讨电针百会、神庭对脂多糖(LPS)引起的系统炎症诱导认知功能障碍的海马抗炎作用机制。方法:健康清洁级成年C57BL/6雄性小鼠45只,体质量18~22 g,采用随机数字法分为对照组、内毒素性脑损伤组(简称模型组)及内毒素性脑损伤+电针组(简称电针组),每组各15只。将小鼠适应饲养1周后,于造模前15 min,电针组给予10 Hz连续波电刺激百会、神庭穴,强度为0.5 mA,15 min后进行造模,模型组和电针组分别腹腔注射LPS(1 mg/kg),对照组给予等容量的溶剂。LPS注射前15 min及24 h后,电针组以同等电刺激进行电针15 min,后行www(what-where-when)实验,记录小鼠对物体的时间、地点及顺序的记忆能力。采用免疫荧光、ELISA等方法,观察小鼠海马组织Iba-1和TNF-α、血浆TNF-α的变化。结果:(1)与对照组比较,模型组小鼠www相关的学习记忆时间减少(P<0.01),外周血(造模后3 h)和海马区(24 h)TNF-α水平显著升高(P<0.01);免疫荧光及ELISA检测表明,模型组小鼠海马区小胶质细胞活化物Iba-1表达显著升高(P<0.01)。(2)与模型组比较,电针组小鼠what和when相关的学习记忆时间增加(P<0.01),外周血(造模后3 h)和海马区(24 h)TNF-α水平下降(P<0.01和P<0.05);免疫荧光及ELISA检测表明,电针组小鼠海马区小胶质细胞活化物Iba-1表达降低(P<0.05)。结论:电针百会、神庭可能通过抑制海马区小胶质细胞活化,降低外周血及海马区炎症因子TNF-α释放,从而改善系统性炎症及其诱导的学习记忆能力下降。Objective:To investigate the hippocampal anti-inflammatory mechanism of electroacupuncture at Baihui and Shenting on cognitive dysfunction induced by Lipopolysaccharide(LPS)intraperitoneal injection.Methods:A total of 45 clean grade adult C57 BL/6 male mice(bodyweight 18-22 g)were randomly divided into control group,endotoxic brain injury group(model group)and endotoxic brain injury+electroacupuncture group(EA group).After one week of adaptive feeding,15 min before LPS injection,the EA group was given 10 Hz electrical stimulation at Baihui and Shenting acupoints,the intensity was 0.5 mA.15 min after electrical stimulation,the model group and EA group were intraperitoneally injected LPS(1 mg/kg),the control group was given equal volume of solvent injection.15 min before LPS injection and 24 h after LPS injection,the EA group was given the same electric stimulation for 15 min,and then the"what-where-when"behavioral test was performed to record the memory ability on the time,place and order of objects.After the behavioral experiment,immunofluorescence and ELISA were used to detect the changes of hippocampal Iba-1,TNF-αand serum TNF-α.Results:1)Compared with the control group,the learning and memory time related to"what-where-when"in the model group was decreased(P<0.01),and serum TNF-α(3 h after LPS)and hippocampal TNF-α(24 h)were significantly increased(P<0.01);immunofluorescence and ELISA detection showed that hippocampal Iba-1 was significantly increased(P<0.01)in the model group.2)Compared with the model group,"what"and"when"learning time in the EA group increased(P<0.01);the serum and hippocampal TNF-αwere obviously decreased(P<0.01 and P<0.05);hippocampal Iba-1 in the EA group was decreased(P<0.05).Conclusion:Electroacupuncture at Baihui and Shenting may improve the cognitive dysfunction caused by systemic inflammation via inhibiting the activation of hippocampal microglia and reducing the release of inflammatory factor TNF-αin the peripheral blood and hippocampal regions.

关 键 词：炎症 认知功能障碍 电针 海马 小胶质细胞 百会 神庭 

分 类 号：R245[医药卫生—针灸推拿学]