LAMP扩增产物检测方法研究进展及基因编辑技术在其中的应用  

Research Progress of LAMP Amplicons Detection Method and the Application of Gene Editing Technology

作　　者：白榕 白琳琳 汪少芸[1] 张芳 BAI Rong;BAI Lin-Lin;WANG Shao-Yun;ZHANG Fang(Fujian Key Laboratory of Inspection and Quarantine Technology Research,College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China)

机构地区：[1]福州大学生物科学与工程学院福建省检验检疫技术研究重点实验室,福州350108

出　　处：《农业生物技术学报》2021年第10期2016-2030,共15页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金面上项目(32172285);福建省自然科学基金面上项目(2019J01239);福建省检验检疫技术研究重点实验室资助项目(FJKF2021-02)。

摘　　要：随着恒温扩增技术的快速发展,扩增产物的检测成为限制该技术快速推广和应用的关键。本文结合环介导恒温扩增技术(loop mediated isothermal amplification, LAMP)特点以及传统检测方法,探究了近几年发展起来的新型产物检测方法,如基于反应体系中副产物生成分析的方法、多重靶标检测为目标的方法,以及利用CRISPR/Cas等的序列特异性的可视化检测方法的原理和应用现状,解析了LAMP产物检测面临的机遇和挑战,旨在为核酸分析产业以及基于核酸扩增分析的应用提供支撑。With the rapid development of isothermalamplification technology, the method to detect amplicons has become the key restricting its rapid popularization and application. Given the characteristics of Loop-mediated isothermal amplification(LAMP) reaction and the current amplicons detection methods, we summarized the progresses which have been made in recent years, including methods based on by-product generation analysis, methods aiming for multiplex detection, and the sequence-specific ones with visual readouts(e. g. CRISPR/Cas based ones). The possible opportunities and challenges are also been discussed.This review provides support for the nucleic acid analysis industry and applications based on nucleic acid amplification.

关 键 词：环介导等温扩增技术(LAMP) 产物检测 核酸 恒温扩增 

分 类 号：S1[农业科学—农业基础科学]