环孢素A对脐带血干细胞和T淋巴细胞的影响  

Impact of cyclosporin A on umbilical cord blood stem cell and T lymphocyte

作　　者：燕法红 居瑞雪 赵云霞[3] 毛晓臻 林彩莲 YAN Fahong;JU Ruixue;ZHAO Yunxia;MAO Xiaozhen;LIN Cailian(Department of Hematology,First Affiliated Hospital of Weifang Medical College,Weifang People’s Hospital,Weifang,261041,China;Department of Laboratory Medicine,First Affiliated Hospital of Weifang Medical College,Weifang People’s Hospital;Department of Obstetrics,First Affiliated Hospital of Weifang Medical College,Weifang People’s Hospital)

机构地区：[1]潍坊医学院第一附属医院潍坊市人民医院血液内科,山东淮坊261041 [2]潍坊医学院第一附属医院潍坊市人民医院检验科 [3]潍坊医学院第一附属医院潍坊市人民医院产科

出　　处：《临床血液学杂志》2021年第8期561-563,567,共4页Journal of Clinical Hematology

基　　金：山东省医药卫生科技发展计划项目(No:2016WS0644)。

摘　　要：目的:探讨环孢素A(CsA)对脐带血干细胞及T淋巴细胞的影响。方法:采集10例产妇的脐带血,每例脐带血分为5份,每份2 mL,设对照组(无CsA)、CsA 5μmol/L组、CsA 10μmol/L组、CsA 20μmol/L组、CsA 40μmol/L组,用血细胞分析仪检测各组的白细胞数,应用流式细胞术检测各组的干细胞(CD34^(+)细胞)、T淋巴细胞(CD3^(+)细胞)占CD45^(+)细胞的比例,并分别计算出干细胞、T淋巴细胞的数值。采用配对样本t检验,分别比较不同浓度的CsA组与对照组在白细胞数、干细胞数、T淋巴细胞数方面的差别。以线性相关分析各组白细胞数与T淋巴细胞数之间的相关性。结果:(1)白细胞数:CsA 5μmol/L组、CsA 10μmol/L组、CsA 20μmol/L组、CsA 40μmol/L组均低于对照组(P<0.05)。(2)干细胞数:CsA 5μmol/L组、CsA 10μmol/L组、CsA 20μmol/L组、CsA 40μmol/L组与对照组比较差异无统计学意义(P>0.05)。(3)T淋巴细胞数:高浓度CsA组(10~40μmol/L)与对照组比较,T淋巴细胞数均降低(P<0.01);低浓度CsA组T淋巴细胞数与对照组差异无统计学意义(P=0.133)。(4)各组内白细胞数与T淋巴细胞数之间无相关性(P>0.05)。结论:CsA无法提高脐带血中的干细胞数,可降低脐带血中的白细胞数和T淋巴细胞数。Objective:To explore the impact of cyclosporin A on umbilical cord blood stem cell and T lymphocyte.Methods:Ten umbilical cord blood units were collected and each was divided into five groups including control group(without CsA),CsA 5μmol/L group,CsA 10μmol/L group,CsA 20μmol/L group and CsA 40μmol/L group with two milliliters for each group.White blood cell(WBC)was measured using automated hematology analyzer.CD34^(+)and CD3^(+)cell ratios were analyzed using flow cytometry.CD34^(+)and CD3^(+)cell counts were further calculated.Paired sample t test was used to compare difference of WBC,stem cell,T lymphocyte between each CsA group and control group respectively.Relationships between WBC and T lymphocyte in each group were analyzed using linear correlation.Results:(1)WBC count was lower in each CsA group compared to control group(P<0.05).(2)There was no difference of stem cell count between each CsA group and control group(P>0.05).(3)T lymphocyte count of CsA 10-40μmol/L group was all lower than that of control group(P<0.01).There was no difference of T lymphocyte count between CsA 5μmol/L group and control group(P=0.133).(4)There was no relationship between WBC and T lymphocyte in each group(P>0.05).Conclusion:CsA can not improve stem cell of umbilical cord blood and can reduce WBC and T lymphocyte.

关 键 词：环孢素A 脐带血 干细胞 T淋巴细胞 白细胞 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R557[医药卫生—基础医学]